Experimental infection by Trypanosoma vivax in goats in the Brazilian semiarid: detection of T. vivax DNA in colostrum and assessment of lactogenic transmission

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Pesquisa Veterinaria Brasileira Pub Date : 2023-02-06 DOI:10.1590/1678-5150-pvb-7119

N. M. Bezerra, T. S. Teófilo, H. N. A. Araújo Júnior, J. B. Silva, G. H. Moura, Kizzy Millenn de Freitas Mendonça Costa, Kaliane Alessandra R. de Paiva, J. S. Batista

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引用次数: 1

Abstract

ABSTRACT: This study aimed to identify the presence of Trypanosoma vivax DNA in the colostrum of infected goats and to explore the possibility of transmission for neonates fed using colostrum collected from infected goats. We used twelve goats in the final third of gestation with an age of approximately 24 months. Six goats were inoculated intravenously with 0.5mL of blood containing approximately 1.25x105 trypomastigotes of T. vivax, and six remained uninfected. The presence of T. vivax in colostrum was evaluated by Polymerase Chain Reaction (PCR). The possibility of T. vivax transmission by colostrum was assessed by feeding six neonates born of serologically negative goats using colostrum from infected goats. Peripheral blood from neonates was collected daily for thirty days to assess the T. vivax presence through the examination of Giemsa-stained smears of leukocyte layers with the buffy coat technique (BCT) and by PCR. The results of a direct examination of colostrum were negative, but PCR confirmed the presence of T. vivax DNA in all infected goats. Additionally, lactogenic transmission by colostrum was not demonstrated once both BCT and PCR of neonate peripheral blood were negative.

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巴西半干旱地区山羊间日锥虫实验感染:初乳中间日锥虫DNA检测及乳源性传播评估

摘要:本研究旨在鉴定感染山羊初乳中间日锥虫DNA的存在，并探讨以感染山羊初乳喂养的新生儿传播间日锥虫的可能性。我们使用了12只在妊娠最后三分之一的山羊，年龄约为24个月。6只山羊静脉注射0.5mL含有约1.25 × 105个间日疟原虫的血液，6只山羊未感染。采用聚合酶链反应(PCR)法测定初乳中间日疟原虫的存在。通过用感染山羊的初乳喂养6只血清学阴性山羊出生的新生儿，评估了间日疟原虫通过初乳传播的可能性。每天收集新生儿外周血，持续30天，通过使用黄皮技术(BCT)和PCR检测吉姆萨染色的白细胞层涂片来评估间日疟原虫的存在。直接检测初乳的结果为阴性，但聚合酶链反应证实在所有感染山羊中都存在间日疟原虫DNA。此外，一旦新生儿外周血的BCT和PCR均为阴性，就不能证明初乳的乳原性传播。

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来源期刊

Pesquisa Veterinaria Brasileira 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

9-18 weeks

期刊介绍： Pesquisa Veterinária Brasileira - Brazilian Journal of Veterinary Research (http://www.pvb.com.br), edited by the Brazilian College of Animal Pathology in partnership with the Brazilian Agricultural Research Organization (Embrapa) and in collaboration with other veterinary scientific associations, publishes original papers on animal diseases and related subjects. Critical review articles should be written in support of original investigation. The editors assume that papers submitted are not being considered for publication in other journals and do not contain material which has already been published. Submitted papers are peer reviewed. The abbreviated title of Pesquisa Veterinária Brasileira is Pesqui. Vet. Bras.