Immunohistochemical analysis of ORF2 protein and ORF3 protein of hepatitis E virus in livers of swine in Mato Grosso state, Brazil

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Pesquisa Veterinaria Brasileira Pub Date : 2022-01-01 DOI:10.1590/1678-5150-pvb-7091

C. G. Campos, Lucas Avelino Dandolini Pavelegini, A. H. Pereira, Marcos A. Souza, C. Pescador

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Abstract

ABSTRACT: Hepatitis E is an emerging zoonotic disease caused by hepatitis E virus (HEV). Immunohistochemistry (IHC) can be used to verify viral presence in human and swine livers. The aim of this study was to comparatively analyze the immunolabeling of the ORF2 protein (pORF2) versus the ORF3 protein (pORF3) of HEV in swine livers from subsistence farms in the state of Mato Grosso, Brazil. This study included 25 liver samples formalin fixed paraffin embedded tissue block from a published molecular detection and immunohistochemistry (IHC) study, which used the HEV pORF3 protein, demonstrating 4% (1/25) of positive immunolabeling and 96% (24/25) negative, in contrast to the molecular exam that showed 24% (6/25) of liver samples positive and 76% (19/25) negative. In order to increase the sensitivity of the IHC technique, these samples were analyzed using the antibody for the detection of HEV pORF2, showing 24% (6/25) immunolabeling positive and 76% (19/25) negative, equivalent to the result of molecular analysis on corresponding samples. Thus, the use of antibody to pORF2 increased the number of HEV cases detectable in the IHC by 600%. The IHC added to molecular techniques can be used as a tool for monitoring viral presence in swine livers, constituting a sensitive diagnostic methodology when liver samples fixed in formalin and embedded in paraffin are available.

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巴西马托格罗索州猪肝脏戊型肝炎病毒ORF2蛋白和ORF3蛋白的免疫组织化学分析

摘要戊型肝炎是由戊型肝炎病毒(HEV)引起的一种新型人畜共患疾病。免疫组织化学(IHC)可用于验证病毒在人和猪肝脏中的存在。本研究的目的是比较分析巴西马托格罗索州自给农场猪肝中HEV的ORF2蛋白(pORF2)和ORF3蛋白(pORF3)的免疫标记。该研究包括25份来自已发表的分子检测和免疫组织化学(IHC)研究的肝脏样本，该研究使用HEV pORF3蛋白，显示4%(1/25)的免疫标记阳性，96%(24/25)的免疫标记阴性，而分子检查显示24%(6/25)的肝脏样本阳性，76%(19/25)的肝脏样本阴性。为了提高免疫组化技术的灵敏度，对这些样品进行HEV pORF2抗体检测，结果显示24%(6/25)免疫标记阳性，76%(19/25)免疫标记阴性，与相应样品的分子分析结果相当。因此，pORF2抗体的使用使免疫组化中检测到的HEV病例数增加了600%。加入分子技术的免疫组化可以作为监测猪肝中病毒存在的工具，当用福尔马林固定并包埋在石蜡中的肝脏样本可用时，构成一种敏感的诊断方法。

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来源期刊

Pesquisa Veterinaria Brasileira 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

9-18 weeks

期刊介绍： Pesquisa Veterinária Brasileira - Brazilian Journal of Veterinary Research (http://www.pvb.com.br), edited by the Brazilian College of Animal Pathology in partnership with the Brazilian Agricultural Research Organization (Embrapa) and in collaboration with other veterinary scientific associations, publishes original papers on animal diseases and related subjects. Critical review articles should be written in support of original investigation. The editors assume that papers submitted are not being considered for publication in other journals and do not contain material which has already been published. Submitted papers are peer reviewed. The abbreviated title of Pesquisa Veterinária Brasileira is Pesqui. Vet. Bras.