HD2 overexpression induces dwarfism and elevated histone deacetylase activities in transgenic Ananas comosus variety MD2

IF 1.2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY
N. A. Halim, B. Tan, N. Khalid, J. S. Yaacob
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引用次数: 0

Abstract

: Plant tissue culture has served as one of the most efficient biotechnological tools to mass produce high quality major crops. Nevertheless, the use of this technique can result in the occurrence of somaclonal variation, which may be brought upon by genetic and/or epigenetic modulations. The epigenetic modulation of plant growth, particularly on the importance of histone deacetylation/acetylation on genome stability, transcriptional regulation, as well as plant development, has been reported in several species such as Arabidopsis , rice, and maize. However, the information on its role in regulating the growth of major crops such as pineapple is still lacking. Previously, we had reported that supplementation of high cytokinin in the tissue culture media and exposure to salinity and abscisic acid (ABA) resulted in the production of somaclonal variants that exhibited dwarf phenotypes, and that these occurrences were associated with epigenetic modulation via the involvement of histone deacetylases (HDACs). Thus, in the present study, we aimed to further elucidate and verify the involvement of histone deacetylation in the occurrence of dwarfism in tissue culture-derived pineapple plants, through HD2 gene overexpression. AtHD2 gene from Arabidopsis thaliana was synthesized and expressed in phenotypically normal Ananas comosus . Transformation efficiency was also studied, and application of 200-µM acetosyringone was observed to be more efficient than 500 µM vanillin by 10%. The transgenic plants were observed to exhibit dwarfed phenotypes and showed significantly high HDAC enzyme activities. Ploidy evaluation of the transgenic plants showed no ploidy change had occurred in the samples and remained as 2n. The relative expression level of AtHD2 was also higher (3.2-fold up-regulation) in the transgenic plants compared to the wild type plants. Collectively, these results indicate that HDACs indeed played an essential role in the response of A. comosus towards abiotic stress, and that this phenomenon is epigenetically regulated.
HD2过表达诱导转基因苋菜MD2矮化和组蛋白去乙酰化酶活性升高
植物组织培养已成为大批量生产优质主要作物的最有效的生物技术手段之一。然而,这种技术的使用可能导致体细胞无性系变异的发生,这可能是由遗传和/或表观遗传调节引起的。植物生长的表观遗传调控,特别是组蛋白去乙酰化/乙酰化对基因组稳定性、转录调控以及植物发育的重要性,已经在拟南芥、水稻和玉米等几种物种中得到了报道。然而,关于它在调节菠萝等主要作物生长中的作用的信息仍然缺乏。在此之前,我们已经报道了在组织培养基中补充高细胞分裂素和暴露于盐度和脱落酸(ABA)会导致产生具有矮化表型的体细胞无性系变异,并且这些发生与通过组蛋白去乙酰化酶(hdac)参与的表观遗传调节有关。因此,在本研究中,我们旨在通过HD2基因过表达进一步阐明和验证组蛋白去乙酰化参与组织培养菠萝植物侏儒症的发生。合成拟南芥中AtHD2基因,并在表型正常的拟南芥中表达。研究了转化效率,发现200µM乙酰丁香酮比500µM香兰素转化效率高10%。转基因植株表型矮化,HDAC酶活性显著提高。对转基因植株进行倍性鉴定,结果表明转基因植株的倍性未发生变化,保持在2n。与野生型相比,转基因植株中AtHD2的相对表达量也更高(上调3.2倍)。综上所述,这些结果表明hdac确实在麻豆对非生物胁迫的反应中发挥了重要作用,并且这种现象是受表观遗传调控的。
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来源期刊
Bragantia
Bragantia AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
2.40
自引率
8.30%
发文量
33
审稿时长
4 weeks
期刊介绍: Bragantia é uma revista de ciências agronômicas editada pelo Instituto Agronômico da Agência Paulista de Tecnologia dos Agronegócios, da Secretaria de Agricultura e Abastecimento do Estado de São Paulo, com o objetivo de publicar trabalhos científicos originais que contribuam para o desenvolvimento das ciências agronômicas. A revista é publicada desde 1941, tornando-se semestral em 1984, quadrimestral em 2001 e trimestral em 2005. É filiada à Associação Brasileira de Editores Científicos (ABEC).
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