Contribution to the Molybdenum Blue Reaction and its Application in Soil Analysi

IF 0.7 Q4 CHEMISTRY, ANALYTICAL
L. Angelova, N. Genova, G. Pencheva, Yasena Statkova, Violeta Yotova, A. Surleva
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引用次数: 0

Abstract

This paper presents a study on the molybdenum blue reaction (MB) as a finishing detection step in soil analysis for quantification of plant available phosphorus. An ammonium acetate/calcium lactate reagent (pH = 4.2) was used for soil phosphorus extraction. The molybdenum blue color reaction using premixed Murphy-Riley reagent and ascorbic acid as a reductant was reinvestigated. UV-Vis characteristics of MB, optimal wavelength, reaction time and concentration of reductant were studied. The effect of ascorbic acid concentration and the reaction time on linearity, bias and uncertainty was discussed. The molybdenum blue reaction was found to obey Beer’s law in the targeted concentration range of 0.04 – 1.0 mg L-1 PO43- - P. The linearity was proved by “lack-of-fit” test. The uncertainty budget was made and the uncertainty was estimated by modelling approach, as well as single laboratory and quality control approach. The recovery and the expanded uncertainty were found to be, respectively, (95.7 ± 8.7) % (P = 95 %, n = 3) and 9.2 mg PO43- - P/kg dry soil (k = 2). The results showed that the soil sample inhomogeneity and the repeatability of extraction process were the main factors which contribute to the uncertainty of measurement in soil analysis.
钼蓝反应的贡献及其在土壤分析中的应用
本文研究了钼蓝反应(MB)作为土壤分析中植物有效磷定量的最后检测步骤。采用pH = 4.2的乙酸铵/乳酸钙试剂提取土壤磷。重新研究了以墨菲-莱利试剂和抗坏血酸为还原剂的钼蓝色反应。研究了MB的紫外可见性、最佳波长、反应时间和还原剂浓度。讨论了抗坏血酸浓度和反应时间对线性、偏置和不确定度的影响。结果表明,在0.04 ~ 1.0 mg L-1 PO43- p的目标浓度范围内,钼蓝反应符合比尔定律,并通过“失拟”试验证明其线性。采用建模法、单实验室法和质量控制法对不确定度进行预算和估计。回收率为(95.7±8.7)% (P = 95%, n = 3),扩展不确定度为9.2 mg PO43- - P/kg干土(k = 2)。结果表明,土壤样品的不均匀性和提取过程的重复性是导致土壤分析测量不确定度的主要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.00
自引率
14.30%
发文量
12
期刊介绍: The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.
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