Validation of the Multiresidue Method Analysis for Pesticides in Bee Honey by UPLC-MS/MS using the Method of Samples Preparation QuEChERS

IF 0.7 Q4 CHEMISTRY, ANALYTICAL

Methods and Objects of Chemical Analysis Pub Date : 2022-01-01 DOI:10.17721/moca.2022.141-152

Y. Omelchun, A. Kobish, N. Klochkova, L. Shevchenko

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Abstract

In this paper we present the results of evaluating the suitability of the developed method for the simultaneous determination of 200 pesticide residues in bee honey using ultraperformance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS). The main stages of extraction of pesticides from the matrix using QuEChERS (Quick Easy Cheap Effective Rugged Safe) sample preparation are described. Appropriate mobile phase composition was used to better identify analytes, internal standards were not applied. The studied list of pesticides is relevant for use in modern agriculture. The limit of quantification of the method (LOQ) for each of the analytes is 0.001 mg/kg, the relative standard deviation in the conditions of repeatability and reproducibility < 20.0 %, the expanded measurement uncertainty is less than the limit of regulatory requirements < 50.0 %. The calibration graph for each of the analytes had a coefficient R2 > 0.99. The use of QuEChERS sample preparation methods allows to reduce labor intensity, time and reagents, increase the accuracy and precision of research in comparison with classical methods of sample preparation. The described method makes it possible to detect a wide range of pesticides and has a fairly high sensitivity

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基于QuEChERS制样方法的超高效液相色谱-质谱联用分析蜜蜂蜂蜜中农药多残留方法的验证

本文对建立的超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定蜂蜜中200种农药残留的适用性进行了评价。介绍了使用QuEChERS(快速、简便、廉价、有效、坚固、安全)样品制备从基质中提取农药的主要步骤。采用合适的流动相组成更好地鉴别分析物，不应用内标。所研究的农药清单与现代农业的使用有关。每种分析物的定量限(LOQ)为0.001 mg/kg，在重复性和再现性条件下的相对标准偏差< 20.0%，扩展测量不确定度小于法规要求的极限< 50.0%。每种分析物的校准图系数R2 > 0.99。与传统的样品制备方法相比，使用QuEChERS样品制备方法可以减少劳动强度、时间和试剂，提高研究的准确性和精密度。所描述的方法使检测范围广泛的农药成为可能，并且具有相当高的灵敏度

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来源期刊

Methods and Objects of Chemical Analysis CHEMISTRY, ANALYTICAL-

CiteScore

1.00

自引率

14.30%

发文量

期刊介绍： The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.