Enhanced Electrochemical Determination Of Riboflavin In Biological And Pharmaceutical Samples At Poly (Arginine) Modified Carbon Paste Electrode

IF 0.7 Q4 CHEMISTRY, ANALYTICAL

Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI:10.17721/moca.2019.216-223

Girish Tigari, J. G. Manjunatha, D. Ravishankar, G. Siddaraju

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引用次数: 8

Abstract

An electrogenerated Polyarginine modified carbon paste electrode (PAMCPE) was fabricated through a simple electropolymerization procedure. The devised electrode was characterized by cyclic voltammetry (CV) and Field Emission Scanning Electron Microscopy (FESEM). This electrode was utilized for electrocatalytic estimation of Riboflavin (RF) and its instantaneous resolution with ascorbic acid (AA) and folic acid (FA) in phosphate buffer solution (PBS) of pH 6.0 by differential pulse voltammetry (DPV). It was observed to be a very responsive electrode for the electrochemical detection and quantification of RF. It was revealed that PAMCPE generates higher current response towards RF contrast to the bare carbon paste electrode (BCPE). Under optimized condition, the RF oxidation current values were linearly reliant on the RF concentration increment with a limit of detection (LOD) of 9.3·10-8 M using DPV. The stable PAMCPE was effectively applied for estimation of RF in B-complex pill and complex human blood serum samples.

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聚精氨酸修饰碳糊电极强化电化学测定生物和药物样品中的核黄素

采用简单的电聚合方法制备了聚精氨酸修饰碳糊电极(PAMCPE)。采用循环伏安法(CV)和场发射扫描电镜(FESEM)对所设计的电极进行了表征。该电极应用差分脉冲伏安法(DPV)在pH为6.0的磷酸缓冲液(PBS)中电催化测定核黄素(RF)及其与抗坏血酸(AA)和叶酸(FA)的瞬时分离度。结果表明，该电极对射频的电化学检测和定量反应非常灵敏。结果表明，与裸碳糊电极(BCPE)相比，PAMCPE对射频产生更高的电流响应。优化条件下，RF氧化电流值与RF浓度增量呈线性关系，DPV检测限为9.3·10-8 M。稳定的PAMCPE可有效地用于b复合物药丸和复杂人血清样品中RF的测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods and Objects of Chemical Analysis CHEMISTRY, ANALYTICAL-

CiteScore

1.00

自引率

14.30%

发文量

期刊介绍： The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.