Carbohydrate, flavonoid, anthocyanin,total phenol, chlorophyll and mineral (K+) content development of wax apple fruit as affected by CPPU and NAA using swabbing technology

Pub Date : 2023-07-28 DOI:10.1590/0100-29452023798
Ahmed Bin Mohamed Sharif Hossain, A. M. Alsaif, H. A. Rudayni, A. Al-hashimi, Mohammad Saad Aleissa, R. M. Taha
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Abstract

Abstract The study was conducted to investigate the effect of CPPU(2-Chloro-4-pyridyl-phenylurea) and NAA (Naphthaleneacetic acid) on the pigments, total phenol, flavonoid and mineral (K+) content in wax apple fruit. CPPU concentrations were 10, 15 and 20ppm (Expt.1) and 6, 12, 18ppm NAA were applied coming after swabbing technique (Expt.2). In Expt.1, the lowest fruit weight, fruit size and chlorophyll content was observed in control treatment. However, the highest fruit weight, fruit size and chlorophyll (SPAD) content was found in 15ppm CPPU. The most effective concentration was 15ppm CPPU for the earlier fruit maturity (color development) compared to other concentrations. Moreover, flavonoid, fructose, inverted sugar, total phenol and K+ were higher in 15ppm CPPU than control, 10 and 20ppm CPPU. In addition, anthocyanin was found increasing trend while developing the fruit maturity represented by color development. In Expt.2, chlorophyll was higher in 15ppm CPPU than control, 6, 18ppm NAA. Furthermore, flavonoid, fructose, inverted sugar, total phenol and K+ content were higher in 12ppm NAA than control, 6 and 18ppm NAA. Besides, the maximum anthocyanin was found in 12ppm NAA. Finally it seemed that 15ppm CPPU and 12ppm NAA were the best concentration for fruit growth and biochemical contents development in wax apple.
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用棉签法测定CPPU和NAA对蜡苹果果实碳水化合物、类黄酮、花青素、总酚、叶绿素和矿物质(K+)含量的影响
摘要本试验研究了2-氯-4-吡啶-苯脲(CPPU)和萘乙酸(NAA)对蜡苹果果实中色素、总酚、类黄酮和矿物质(K+)含量的影响。CPPU浓度分别为10、15和20ppm(实验1),NAA浓度分别为6、12、18ppm(实验2)。在试验1中,对照处理的单果重、果实大小和叶绿素含量最低。果实重、果实大小和叶绿素(SPAD)含量以15ppm的CPPU最高。与其他浓度相比,15ppm CPPU对早熟果实(颜色发育)最有效。15ppm CPPU中黄酮类化合物、果糖、倒糖、总酚和K+含量均高于对照、10和20ppm CPPU。花青素在果实成熟期呈上升趋势,以颜色发育为代表。在实验2中,15ppm CPPU的叶绿素含量高于对照、6,18 ppm NAA。12ppm NAA处理的黄酮类、果糖、倒糖、总酚和K+含量均高于对照、6和18ppm NAA处理。花青素在12ppm NAA时含量最高。结果表明,15ppm的CPPU和12ppm的NAA是促进蜡苹果果实生长和生化物质发育的最佳浓度。
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