The Effect of Short Term Alkalinity on Photosynthesis Apparatus in Soil Cyanobacterium Fischerella sp. FS 18 - Ecophysiological Approach

Q4 Agricultural and Biological Sciences

International Journal on Algae Pub Date : 2023-01-01 DOI:10.1615/interjalgae.v25.i1.60

Fatemeh Yadollahi, S. Shokravi, M. Mahmoudjanlo, M. Ebadi

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引用次数: 0

Abstract

The possibility of change in the phycobilisome status, photosynthetic pigments, ratios and light parameters of soil cyanobacteria Fischerella sp. FS 18 has been investigated by two pre-treatment factors, with neutral and extreme alkaline pH (7 and 9) over a short period. After purification, cyanobacteria were subjected to extreme alkaline treatment for under one hour at 20 min intervals (20, 40 and 60 min). A sample was selected as a control without pre-treatment. Colorimetric assays (phycocyanin, alophycocyanin, phycoerythrin, chlorophyll) and a comparison of the combined effect of time and alkalinity on photosystemic ratios were performed through photophysiology. The photosynthesis - light curves were compared with direct measurements. The results showed that the combination of time and alkalinity after 20 min of inoculation significantly increased the performance of the photosystem and stability of the phycobilin system. The 40 min time interval, under both neutral and alkaline conditions, significantly increased the yield of photosystem II, increased the production of the first photosystem compared to the second system, increased the linear fraction of the photosynthesis-light curve, and reduced the energy needed to achieve maximum photosynthesis. Maximal photosynthesis was entirely different at 40 min pretreatment conditions versus without pretreatment. Concerning the 20 and 60 min time intervals, the results show no specific regularity or trend. Production of light collecting antennas is influenced by both time and alkalinity. Two important conclusions from this study include: applying pre-treatment times, even at 60 min or less, cause a significant change in the structure and performance of the photosynthetic apparatus; as well as,-using simple alkaline pretreatments in a very short time period, saving energy whilst, photosynthesis and cyanobacterial materialization can be significantly increased.

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短期碱度对土壤蓝藻Fischerella sp. fs18光合机制的影响——生态生理学方法

研究了中性pH和极碱性pH(7和9)短时处理对土壤蓝藻Fischerella sp. FS 18藻胆酶体状态、光合色素、比例和光照参数变化的影响。纯化后，对蓝藻进行极碱性处理，每隔20分钟(20、40和60分钟)，处理时间不到1小时。选择一份样品作为不经预处理的对照。通过光生理学进行比色测定(藻蓝蛋白、藻蓝蛋白、藻红蛋白、叶绿素)，并比较时间和碱度对光系统比率的综合影响。并与直接测量结果进行了比较。结果表明，接种20 min后，时间和碱度的结合显著提高了藻胆素系统的光系统性能和稳定性。在中性和碱性条件下，40 min的时间间隔显著提高了光系统II的产量，与第二光系统相比，第一光系统的产量增加，光合作用-光曲线的线性分数增加，实现最大光合作用所需的能量减少。预处理40 min与未预处理的最大光合作用完全不同。对于20和60分钟的时间间隔，结果没有显示出特定的规律或趋势。集光天线的产生受时间和碱度的影响。本研究的两个重要结论包括:施用预处理时间，即使在60分钟或更短的时间内，也会导致光合机构的结构和性能发生显著变化;此外，在很短的时间内使用简单的碱性预处理，可以节约能源，同时可以显著增加光合作用和蓝藻物化。

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来源期刊

International Journal on Algae Agricultural and Biological Sciences-Plant Science

CiteScore

0.40

自引率

0.00%

发文量

期刊介绍： The algae are heterogeneous assemblage of phytosynthetic organisms, one of the most vast and diverse groups of ancient photoautotrophic pro- and eukaryotic organisms (about 30 000 known species). They are micro- and macroscopic, unicellular, colonial, or multicellular, mobile and immobile, attached and free-living. Algae are widespread in water and soil habitats, at different geographic latitudes, and on all continents. They occur in waters with different degrees of salinity, trophicity, organic matter, and hydrogen ions, and at various temperatures. They include planktonic, periphytonic and benthic organisms. Algae are unique model organisms in evolutionary biology and also are used in various genetic, physiological, biochemical, cytological, and other investigations.