Absorption of FD-150 into Intestinal Cells by Microplasma

Q3 Physics and Astronomy

Plasma Medicine Pub Date : 2022-01-01 DOI:10.1615/plasmamed.v12.i4.20

J. Krištof, Ryo Yokoyama, A. Yahaya, S. A. Rimi, M. Blajan, Kazuo Shimizu

引用次数: 0

Abstract

Introduction of molecules into the cells is part of gene transfection or cell-mediated drug delivery. The molecules can effectively decrease side effects of the drug and increase their targeting. Air microplasma discharge was used to treat rat intestinal cells to induce absorption of fluorescein isothiocyanate-dextran with a molecular weight of 150 kDa (FD-150). Absorption of FD-150 was confirmed by observation using fluorescence microscope. Efficiency of absorbance was estimated by a plate reader, and the viability of the cells was measured by a cell counter. The viability of the cells was proportional to the decrease in treatment time and the increase in treatment distance from the microplasma electrode. The absorption of FD-150 was found to be proportional to the increase in time and distance. No improvement in absorption or viability was observed during the incubation time. Cell treatment by the plasma-treated medium showed a lower absorption of FD-150 than direct treatment but the same cell viability. Concentration of ozone in the gas phase and OH radical in the liquid phase were observed.

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微等离子体对FD-150在肠细胞中的吸收

将分子引入细胞是基因转染或细胞介导的药物传递的一部分。这些分子可以有效地减少药物的副作用，增加药物的靶向性。采用空气微等离子体放电诱导大鼠肠细胞吸收分子量为150kda的异硫氰酸-葡聚糖荧光素(FD-150)。荧光显微镜下观察FD-150的吸收情况。吸收效率由平板阅读器估计，细胞活力由细胞计数器测量。细胞活力与处理时间的缩短和与微等离子体电极处理距离的增加成正比。发现FD-150的吸收与时间和距离的增加成正比。在孵育期间，没有观察到吸收或活力的改善。经等离子体处理的细胞对FD-150的吸收低于直接处理，但细胞存活率相同。观察了气相臭氧浓度和液相OH自由基浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasma Medicine Physics and Astronomy-Physics and Astronomy (all)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Technology has always played an important role in medicine and there are many journals today devoted to medical applications of ionizing radiation, lasers, ultrasound, magnetic resonance and others. Plasma technology is a relative newcomer to the field of medicine. Experimental work conducted at several major universities, research centers and companies around the world over the recent decade demonstrates that plasma can be used in variety of medical applications. It is already widely used surgeries and endoscopic procedures. It has been shown to control properties of cellular and tissue matrices, including biocompatibility of various substrates. Non-thermal plasma has been demonstrated to deactivate dangerous pathogens and to stop bleeding without damaging healthy tissue. It can be used to promote wound healing and to treat cancer. Understanding of various mechanisms by which plasma can interact with living systems, including effects of reactive oxygen species, reactive nitrogen species and charges, has begun to emerge recently. The aim of the Plasma Medicine journal will be to provide a forum where the above topics as well as topics closely related to them can be presented and discussed. Existing journals on plasma science and technology are aimed for audiences with primarily engineering and science background. The field of Plasma Medicine, on the other hand, is highly interdisciplinary. Some of prospective readers and contributors of the Plasma Medicine journal are expected to have background in medicine and biology. Others might be more familiar with plasma science. The goal of the proposed Plasma Medicine journal is to bridge the gap between audiences with such different backgrounds, without sacrificing the quality of the papers be their emphasis on medicine, biology or plasma science and technology.