Enhancement of PC12 Neurite Extension via Plasma-Activated Medium by Nonthermal Atmospheric-Pressure Plasma-Bubbling System

Q3 Physics and Astronomy

Plasma Medicine Pub Date : 2019-01-01 DOI:10.1615/plasmamed.2020033059

Takanobu Haccho, Atsuru Kanno, H. Ichikawa, Koji Yamamoto, Y. Morita, E. Nakamachi

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引用次数: 1

Abstract

In this study, an axonal extension enhancement system was developed using plasma-activated medium (PAM), which was generated through a nonthermal atmospheric-pressure plasma-bubbling system. Until recently, a number of regeneration methods for treatment of an injured central nervous system (CNS) have been proposed and examined. However, problems remain regarding quick recovery. It is also strongly required to establish an efficacious regeneration method for an injured CNS. Therefore, this study aimed to enhance the nerve axonal extension using PAM. The effectiveness of PAM in enhancing axonal extension was evaluated. First, the amounts of reactive species, such as H2O2, NO2, and NO3, in PAM were measured. Second, the stimulating effect of PAM was investigated by morphologically evaluating the PC12 cells after PAM stimulation. Experiments were conducted under 20 conditions to determine an optimal condition. Furthermore, the mechanism of enhancing PC12 neurite extension using PAM was elucidated. It was found that the Ca2+ concentration in PC12 cells was increased upon PAM stimulation and that Erk and CREB were activated, which enhanced the neurite extension. The results show that the PAM exhibited a selective cell-activation property for the treatment of CNS injuries.

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非热大气压等离子体鼓泡系统增强等离子体活化介质对PC12神经突延伸的影响

在本研究中，利用等离子体活化介质(PAM)开发了一种轴突扩展增强系统，该系统是通过非热大气压等离子体鼓泡系统产生的。直到最近，许多治疗中枢神经系统(CNS)损伤的再生方法被提出和研究。然而，在快速恢复方面仍然存在问题。建立一种有效的中枢神经系统损伤再生方法也是迫切需要的。因此，本研究旨在利用PAM增强神经轴突伸展。评价了PAM增强轴突延伸的效果。首先，测定PAM中H2O2、NO2、NO3等活性物质的含量。其次，通过形态学评价PAM刺激后PC12细胞的刺激作用。在20种条件下进行了实验，以确定最佳条件。进一步阐明了PAM增强PC12神经突延伸的作用机制。结果发现，PAM刺激PC12细胞后Ca2+浓度升高，Erk和CREB被激活，神经突伸展增强。结果表明，PAM对中枢神经系统损伤具有选择性的细胞活化作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasma Medicine Physics and Astronomy-Physics and Astronomy (all)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Technology has always played an important role in medicine and there are many journals today devoted to medical applications of ionizing radiation, lasers, ultrasound, magnetic resonance and others. Plasma technology is a relative newcomer to the field of medicine. Experimental work conducted at several major universities, research centers and companies around the world over the recent decade demonstrates that plasma can be used in variety of medical applications. It is already widely used surgeries and endoscopic procedures. It has been shown to control properties of cellular and tissue matrices, including biocompatibility of various substrates. Non-thermal plasma has been demonstrated to deactivate dangerous pathogens and to stop bleeding without damaging healthy tissue. It can be used to promote wound healing and to treat cancer. Understanding of various mechanisms by which plasma can interact with living systems, including effects of reactive oxygen species, reactive nitrogen species and charges, has begun to emerge recently. The aim of the Plasma Medicine journal will be to provide a forum where the above topics as well as topics closely related to them can be presented and discussed. Existing journals on plasma science and technology are aimed for audiences with primarily engineering and science background. The field of Plasma Medicine, on the other hand, is highly interdisciplinary. Some of prospective readers and contributors of the Plasma Medicine journal are expected to have background in medicine and biology. Others might be more familiar with plasma science. The goal of the proposed Plasma Medicine journal is to bridge the gap between audiences with such different backgrounds, without sacrificing the quality of the papers be their emphasis on medicine, biology or plasma science and technology.