Dendrobium nobile in vitro flowering induction

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-07-31 DOI:10.1590/2447-536x.v29i2.2612

M. Nadal, Gracielle Vidal Silva Andrade, José Henrique Nunes Flores, Michele Valquíria dos Reis, Vantuil Antonio Rodrigues, M. Pasqual

引用次数: 0

Abstract

Abstract In vitro flowering is a technique used in genetic improvement that accelerates generations and favors the faster fixation of new traits of agronomic and market interest. The study aimed to establish a protocol for the in vitro flowering of Dendrobium nobile, through the combined temperature and of the growth regulator thidiazuron (TDZ) effects. Experiments was performed in a flask that was kept in a BOD incubator at 18, 21, or 24 °C or in a growth room at 26 °C. The TDZ concentrations were 0.0, 0.5, 1.0, and 2.0 mg L-1. The highest percentage of flowering shoots of 13.7% occurred at a concentration of 2 mg L-1 of TDZ grown at a temperature of 18 °C. Dendrobium nobile in vitro flowering was promoted in plants cultivated in ½ MS supplemented with 30 g L-1 sucrose, 5.5 g L-1 agar, 100 mL L-1 coconut water, pH 6.0, and 2.0 mg L-1 TDZ at a controlled temperature of 18 °C and a photoperiod of 16 h. The in vitro flowering induction protocol of the D. nobile species could be used or improved for future studies.

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石斛离体开花诱导

体外开花是一种用于遗传改良的技术，它可以加速世代，有利于更快地固定农艺和市场兴趣的新性状。本研究旨在通过温度和生长调节剂噻脲(TDZ)的联合作用，建立石斛(Dendrobium nobile)的离体开花方案。实验在烧瓶中进行，烧瓶保存在BOD培养箱中，温度为18、21或24°C，或在生长室中，温度为26°C。TDZ浓度分别为0.0、0.5、1.0、2.0 mg L-1。TDZ浓度为2 mg L-1，温度为18°C时，开花芽率最高，为13.7%。在1 / 2 MS培养基中，添加30 g L-1蔗糖、5.5 g L-1琼脂、100 mL L-1椰子水、pH 6.0和2.0 mg L-1 TDZ，控制温度为18℃，光周期为16 h，可促进石斛离体诱导开花。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.