Mobilized Mesenchymal Stem Cells for Tissue Engineering in Preclinical Models

Abstract

Background: Mesenchymal Stem Cells obtained from bone marrow are an alternative source of cells for tissue engineering. More studies are done with Mobilized Peripheral Blood Cells (MPBMSC) due to their plasticity on being able to be differentiated in mesenchymal tissues. Objective:The objective of this study was to standarize the mobilization, isolation, and characterization process of mesenchymal stem cells obtained from peripheral blood in an animal model. Methods: Four Suffolk sheep with ages of 2 years were mobilized using granulocyte colony-stimulating factor (G-CSF). Administration dose was 10mg per kilogram of body weight, applied in subcutaneous area every 24 hours, in three consecutive days. The mobilized cells were characterized by an antibody panel for surface markers (CD105, CD90, CD73, CD34, and CD45) and cultured in monolayer during 3 weeks. Results: All population of leucocytes cells have similar behavior during day 1 to day 4. We observed an improvement of populations at day 2 with maximum elevation at day 3 and decline at day 4 in the mobilized peripheral blood. Plastic adhesion and fibroblastic like morphology was observed in mobilized cells. Conclusions: Is feasible to increase the mesenchymal stem cells number in peripheral blood with the three day administration of G-CSF. Isolated cells expressed the characteristic markers of mesenchymal stem cells by flow citometry.