Synergistic effect of benzylaminopurine and meta-Topolin combination for micropropagation of gerbera ‘Pink Melody’

IF 1.2 4区农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY

Ciencia E Agrotecnologia Pub Date : 2022-01-01 DOI:10.1590/1413-7054202246017521

Aaqib Shaheen, Y. Dewir, Mafatlal M. Kher, Majid Khan, A. El-Banna, Ahmed Alaizari

引用次数: 2

Abstract

ABSTRACT Gerbera (Gerbera jamesonii Bolus ex Hook. F.; Asteraceae), is one of the most economically important ornamental plants due to its aesthetic value. In the present study, we established a micropropagation method for the large-scale production of quality planting material of gerbera ‘Pink Melody’. Eighty-six percent of the capitulum explants produced adventitious shoots (15.44 ± 0.34 shoots per capitulum) on Murashige and Skoogs (MS) medium containing 2 mg L−1 6-benzylaminopurine (BAP) after six weeks of incubation. The highest shoot multiplication rate (17 shoots per explant) was obtained on MS medium supplemented with BAP and meta-Topolin (each at 2 mg L−1) after eight weeks. The micro-shoots were successfully rooted (91.35%) on half-strength MS medium containing 2 mg L−1 indole-3-butyric acid (IBA) within four weeks. The micropropagated plantlets were acclimatized with a 97.5% survival rate and produced flowers with no visible morphological aberrations.

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苄氨基嘌呤与间topolin组合对非洲菊‘粉红旋律’微繁的协同效应

非洲菊(Gerbera jamesonii Bolus ex Hook)。f;菊科植物(Asteraceae)，因其独特的观赏价值而成为经济上最重要的观赏植物之一。本研究建立了非洲菊‘粉红旋律’优质种材规模化生产的微繁方法。86%的头状花序外植体在含有2mg L−1 6-苄基胺嘌呤(BAP)的MS培养基上培养6周后产生不定芽(15.44±0.34个头状花序)。在添加BAP和topolin(各2 mg L−1)的MS培养基上，8周后的芽增殖率最高(17个芽/外植体)。在含2 mg L−1吲哚-3-丁酸(IBA)的半强MS培养基上，微芽在4周内生根成功(91.35%)。小苗驯化成活率为97.5%，开花无明显形态畸变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ciencia E Agrotecnologia 农林科学-农业综合

CiteScore

2.30

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： A Ciência e Agrotecnologia, editada a cada 2 meses pela Editora da Universidade Federal de Lavras (UFLA), publica artigos científicos de interesse agropecuário elaborados por membros da comunidade científica nacional e internacional. A revista é distribuída em âmbito nacional e internacional para bibliotecas de Faculdades, Universidades e Instituições de Pesquisa.