Various vigour test methods to rank seed lot quality and predict field emergence in two forage grasses

Abstract

The development of seed vigour testing methods is important for high seed quality selection and, consequently, ensuring high seedling emergence and uniform establishment in the field, particularly in harsh environments. In this study, standard germination (SG), germination under water and temperature stress conditions, radicle emergence (RE) count, accelerated ageing (AA), and electrical conductivity (EC) tests were applied to evaluate the vigour of Poa crymophila and Elymus dahuricus seed lots. SG ranged from 70 to 88% for P. crymophila and 81 to 97% for E. dahuricus, with no significant correlation with field emergence (FE) for either species. EC was significantly correlated (r = -0.774, P < 0.05) with FE for P. crymophila seeds, but not for E. dahuricus. RE counts at 144 and 108 hours from sowing for P. crymophila and E. dahuricus, respectively, were significantly correlated with FE. Germination after one day of AA for P. crymophila and four days for E. dahuricus were more closely correlated with FE than other AA periods. Germination at -0.2 MPa and 15°C for P. crymophila, 0.0 MPa and 20°C for E. dahuricus also related to field emergence.