Icosiella neglecta (Nematoda, Onchocercidae) in Ukraine: Occurrence, Hosts, Morphological and Molecular Characterisation

Q3 Agricultural and Biological Sciences
Y. Kuzmin, I. Dmytriieva, R. Svitin
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Abstract

Icosiella neglecta (Diesing, 1851) is a filarial nematode infecting anuran amphibians in the western Palaearctic. In the present survey, we found I. neglecta in 27 out of 28 localities in eight regions of Ukraine. In those localities, 56.5 % of sampled green frogs (Pelophylax esculentus complex) were infected. The infection prevalence was similar in the two parental species, P. ridibundus (Pallas) and P. lessonae (Camerano), and their hybrid form, P. esculentus (Linnaeus), while the infection intensity was comparatively higher in P. ridibundus, as well as in the samples including both P. ridibundus and P. esculentus. Morphological examination of 40 females and 80 males of I. neglecta confirmed the consistency of main qualitative characters, whereas most metrical characters appeared to be rather variable and related to body length. Muscular oesophagus dimensions and spicule length in males were found to be the least variable. In the studied sample of males, 54 % had a longer spicule on the left side and a shorter one on the right side; the other 46 % had a longer spicule on the right side. Our molecular study using mitochondrial COI gene sequences confirmed the strong genetic similarity of I. neglecta populations in Ukraine and most other parts of its distribution area. A single nuclear 18S sequence generated was identical to one previously deposited in GenBank, originating from Ukraine.
乌克兰忽略盘尾丝虫病线虫(线虫,盘尾丝虫科):发生、寄主、形态和分子特征
忽略Icosiella neglect (dising, 1851)是一种感染古北西部无脊椎两栖动物的丝状线虫。在目前的调查中,我们在乌克兰8个地区的28个地区中发现了27个地区的I.忽视。疫区绿蛙(Pelophylax esculentus complex)感染率为56.5%。在两种亲本种(帕拉斯)和小角斑蝶(卡梅拉诺)及其杂交形式斑蝶(林奈)中,侵染率相似,但斑蝶侵染强度较高,斑蝶和斑蝶侵染强度均较高。对40株雌株和80株雄株的形态检验证实了主要质量性状的一致性,而大多数格律性状表现出较大的变异,且与体长有关。肌肉食道尺寸和针状体长度在男性中变化最小。在研究的男性样本中,54%的人左侧的针状体较长,右侧的针状体较短;另外46%的人右侧有较长的针状体。我们利用线粒体COI基因序列进行的分子研究证实,乌克兰及其分布区域的大部分其他地区的忽略蠓种群具有很强的遗传相似性。生成的单个核18S序列与先前存放在GenBank中的来自乌克兰的序列相同。
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来源期刊
Zoodiversity
Zoodiversity Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
1.20
自引率
0.00%
发文量
29
审稿时长
8 weeks
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