Tri-Lineage Differentiation of NTERA2 Clone D1 Cells towards Neural, Hepatic and Osteogenic Lineages in Vitro.

IF 0.8 4区生物学 Q4 BIOLOGY

Folia Biologica-Krakow Pub Date : 2021-01-01 DOI:10.14712/fb2021067050174

Balázs Széky, B. Mayer, M. Gyongy, A. Hajdara, S. Barsi, S. Kárpáti, K. Nemeth

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Abstract

Over the past decades, the in vitro use of pluripotent cell lines gained a crucial role in toxicology, preclinical drug testing and developmental biology. NTERA2 clone D1 cells were identified as pluripotent cells with high potential for neural differentiation. Although they are commonly used cellular sources in neuropharmacology and neurodevelopmental studies, their endodermal and mesodermal differentiation potential awaits further characterization. Here, we devised improved protocols for hepatogenic and osteogenic differentiation of NTERA2 clone D1 cells. Our in vitro differentiation assays showed significant up-regulation of multiple hepatogenic markers. We also observed robust mineralization and osteogenic marker expression of NTERA2 clone D1 cells upon in vitro osteogenic induction. These results suggest that NTERA2 clone D1 cells may be utilized as an in vitro model system to study various aspects of liver biology and osteogenesis. In addition, tri-lineage differentiation of NTERA2 clone D1 cells may serve as a simple experimental control system when validating pluripotency of other cell types.

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NTERA2克隆D1细胞向神经、肝和成骨三系的体外分化

在过去的几十年里，体外使用多能细胞系在毒理学、临床前药物测试和发育生物学中发挥了至关重要的作用。NTERA2克隆D1细胞被鉴定为具有高神经分化潜能的多能细胞。虽然它们是神经药理学和神经发育研究中常用的细胞来源，但它们的内胚层和中胚层分化潜力有待进一步表征。在这里，我们设计了改进的NTERA2克隆D1细胞的肝源性和成骨性分化方案。我们的体外分化实验显示多种肝源性标志物显著上调。我们还观察到在体外成骨诱导下，NTERA2克隆D1细胞的矿化和成骨标志物表达强劲。这些结果表明，NTERA2克隆D1细胞可以作为一个体外模型系统来研究肝脏生物学和成骨的各个方面。此外，NTERA2克隆D1细胞的三系分化可以作为验证其他细胞类型多能性的简单实验控制系统。

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来源期刊

Folia Biologica-Krakow 医学-生物学

CiteScore

1.10

自引率

14.30%

发文量

审稿时长

>12 weeks

期刊介绍： Folia Biologica (Kraków) is an international online open access journal accepting original scientific articles on various aspects of zoology: phylogeny, genetics, chromosomal studies, ecology, biogeography, experimental zoology and ultrastructural studies. The language of publication is English, articles are assembled in four issues per year.