Optical Properties of Lentil Seed Coats Using Fiber-Optic Spectroscopy

IF 0.8 4区农林科学 Q4 AGRICULTURAL ENGINEERING

Applied Engineering in Agriculture Pub Date : 2022-01-01 DOI:10.13031/aea.14456

Nsuhoridem I. Jackson, A. Vandenberg, M. Subedi, S. Noble

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Abstract

HighlightsA fiber-optic spectroscopy system was set up and validated for measurement of optical properties of lentil seed coat.Light transmission properties of major lentil seed coat types were measured to evaluate their protective ability.Light transmission was detected mainly in the UVA and visible regions in all seed coat types except zero tannin.The protective abilities differed depending on seed coat type (important in breeding for cotyledon protection).Abstract. The light transmission of 20 lentil genotypes, representing six color classes (black, green, tan, brown grey, and zero tannin) was investigated to determine the degree to which seed coats prevent light transmission and possible photodegradation of cotyledon color. A fiber-optic spectroscopy system was set up for measurement; it consisted a deuterium-halogen light source, spectrometer, fiber-optic incidence and transmission probes, and sample holders. The nadir-aligned transmission spectra were measured in wavelength ranges of 250 to 850 nm. To study variability in light transmission of the different seed coat types, the curves were integrated in three wavelength regions to obtain Cumulative UV Transmission (CUVT, 250 to 400 nm), Cumulative VIS Transmission (CVIST, 401 to 700 nm), and Cumulative NIR Transmission (CNIRT, 701 to 850 nm), respectively. Tests for significant differences in light transmission were then done using analysis of variance (ANOVA) via General Linear Modelling and Posthoc GLM Tukey tests. Results showed that all the seed coat types, except zero tannin, showed no detectable transmission of UV light from 250 to 315 nm. Black seed coats showed detectable transmission from 615 to 850 nm, while other seed coat types transmitted varying percentages of light from 315 to 850 nm. The results of ANOVA showed that there were significant (p<0.05) differences in light transmission properties of the major seed coat types. Keywords: Fiber optics, Lentils, Light transmission, Pulse quality, Spectroscopy, Seed coat.

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小扁豆种皮光学特性的光纤光谱分析

建立并验证了一种用于测定小扁豆种皮光学特性的光纤光谱系统。测定了主要小扁豆种皮类型的透光性能，评价了其防护能力。除单宁含量为零外，所有种皮主要在UVA区和可见光区进行透光检测。不同种皮类型对子叶的保护能力不同(种皮类型对育种保护子叶很重要)。研究了6种颜色(黑色、绿色、棕褐色、棕灰色和无单宁)的20个小扁豆基因型的透光性，以确定种皮对子叶颜色的透光性和可能的光降解程度。建立了光纤光谱测量系统;它由氘卤素光源、光谱仪、光纤入射和透射探头以及样品夹组成。在250 ~ 850 nm的波长范围内测量了最低点对准的透射光谱。为了研究不同种皮类型的光透射变异性，对3个波长区域的曲线进行积分，分别得到累积UV透射率(CUVT, 250 ~ 400 nm)、累积VIS透射率(CVIST, 401 ~ 700 nm)和累积NIR透射率(CNIRT, 701 ~ 850 nm)。然后通过一般线性模型和postthoc GLM Tukey检验，使用方差分析(ANOVA)对光透射率的显着差异进行检验。结果表明，除单宁含量为零外，所有种皮在250 ~ 315 nm范围内均无紫外透射。黑色种皮在615 ~ 850nm范围内的透射率可检测到，而其他种类的种皮在315 ~ 850nm范围内的透射率不同。方差分析结果表明，主要种皮类型的透光性差异显著(p<0.05)。关键词:光纤，扁豆，光透射，脉冲质量，光谱学，种皮

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来源期刊

Applied Engineering in Agriculture 农林科学-农业工程

CiteScore

1.80

自引率

11.10%

发文量

审稿时长

6 months

期刊介绍： This peer-reviewed journal publishes applications of engineering and technology research that address agricultural, food, and biological systems problems. Submissions must include results of practical experiences, tests, or trials presented in a manner and style that will allow easy adaptation by others; results of reviews or studies of installations or applications with substantially new or significant information not readily available in other refereed publications; or a description of successful methods of techniques of education, outreach, or technology transfer.