Glycation of Albumin and Gamma-Globulin in Vitro

Abstract

In order to estimate the fructosamine level in plasma we measure fructoselysine, which consists of glucose bound to a lysine residue of serum proteins. Thus, it is possible that the fructosamine level can be affected by the level of serum proteins. Therefore, an in vitro study was performed to elucidete the relationship between fructosamine level and the concentrations of albumin and gamma-globulin. The incubation of albumin and gamma-globulin with varying concentrations of glucose showed that the levels of fructosamine increased in proportion to the concentrations of albumin and gamma-globulin. At given concentrations of albumin and gamma-globulin, the level of fructosamine increased with the concentration of glucose. The glycation of gammaglobulin was found in much lower concentration than of albumin. It was suggested that influence of glycated gamma-globulin can be ignored during fructosamine assay.