The lack of acute effect of low-to-moderate-dose ionising radiation on endothelial proteome

Q4 Medicine

International Journal of Low Radiation Pub Date : 2013-08-01 DOI:10.1504/IJLR.2013.055606

R. Nylund, Elina Lemola, E. Eklund, A. Hakanen, D. Leszczyński

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引用次数: 2

Abstract

Epidemiological studies have shown that moderate and low radiation doses to the heart area may result in an increase in cardiovascular mortality. It has been suggested that cardiovascular endothelium could be one of the targets for the ionising radiation-induced heart injury. We have used human endothelial cells as an in vitro model to study immediate effects of ionising radiation on endothelium. Cells were exposed to low and moderate doses of γ-radiation, and short-term protein expression profiles were examined. Proteomics analysis did not show significant changes in the examined protein expression profiles after the γ-irradiation in any of the examined conditions. The molecular level cellular damage was verified by examining phosphorylation of tumour suppressor p53 binding protein 1, which was dose- and time-dependent. Further examination of cellular proteome and phosphoproteome, using more sensitive quantification and detection techniques, is warranted and might reveal changes, which were not detected in this study.

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低至中剂量电离辐射对内皮蛋白组缺乏急性效应

流行病学研究表明，对心脏部位的中等和低剂量辐射可能导致心血管疾病死亡率增加。研究表明，心血管内皮可能是电离辐射致心脏损伤的靶点之一。我们用人内皮细胞作为体外模型来研究电离辐射对内皮细胞的直接影响。将细胞暴露于低剂量和中等剂量的γ-辐射中，并检测短期蛋白表达谱。蛋白质组学分析显示，在任何检查条件下，γ辐照后所检查的蛋白质表达谱没有显着变化。通过检测肿瘤抑制因子p53结合蛋白1的磷酸化，证实了分子水平的细胞损伤，这是剂量和时间依赖性的。进一步检查细胞蛋白质组和磷蛋白质组，使用更敏感的定量和检测技术，是必要的，并可能揭示变化，这在本研究中未被检测到。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis

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期刊介绍： The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.