An in vitro evidence for caffeic acid, rosmarinic acid and trans cinnamic acid as a skin protectant against γ-radiation

Q4 Medicine

International Journal of Low Radiation Pub Date : 2014-07-15 DOI:10.1504/IJLR.2014.063414

F. Hakkim, Miwa Miura, N. Matsuda, Ahmed Suleiman Alharassi, G. Guillemin, M. Yamauchi, G. Arivazhagan, Hyuk Song

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引用次数: 13

Abstract

Ionising radiation (γ or X-rays) is the mandatory tool to treat cancer despite its detrimental effects in particular on skin cells which lead to severe dermatological diseases and carcinogenesis. Natural antioxidants caffeic acid (CA), rosmarinic acid (RA), trans cinnamic acid (TCA), p-coumaric acid (PCA), and hydroxyphenyllactic acid (HPA) acid are known to be potent anticancer and antioxidant agents. Current study is designed to provide experimental evidence as these compounds offer radiation protection for skin cells. Non-toxic concentrations of CA, RA, TCA, PCA, and HPA were tested for radiation protection, γ-radiation induced intracellular reactive oxygen species (ROS) by flow cytometry and DNA double strand break in human keratinocytes (HaCaT cells) by immunocytochemistry. CA, RA and TCA pretreatment can protect the HaCaT cells by 40%, 20%, 15% respectively through scavenging γ-radiation induced ROS and decreasing number of post irradiation 53bp1 foci. Inclusion of these compounds in chemo-radiother...

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咖啡酸、迷迭香酸和反式肉桂酸作为抗γ辐射皮肤保护剂的体外证据

电离辐射(γ或x射线)是治疗癌症的强制性工具，尽管其有害影响，特别是对皮肤细胞，导致严重的皮肤病和致癌。天然抗氧化剂咖啡酸(CA)、迷迭香酸(RA)、反式肉桂酸(TCA)、对香豆酸(PCA)和羟基苯基乳酸(HPA)酸被认为是有效的抗癌和抗氧化剂。目前的研究旨在提供实验证据，因为这些化合物对皮肤细胞具有辐射保护作用。采用流式细胞术检测无毒浓度CA、RA、TCA、PCA和HPA的辐射防护和γ辐射诱导的细胞内活性氧(ROS)，免疫细胞化学检测人角化细胞(HaCaT细胞)DNA双链断裂。CA、RA和TCA预处理通过清除γ辐射诱导的ROS和减少照射后53bp1病灶数量，对HaCaT细胞的保护作用分别为40%、20%和15%。这些化合物包含在化学放射…

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来源期刊

International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis

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期刊介绍： The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.