Radiobiology worker risk assessment using stress indicators and proteomics

Q4 Medicine

International Journal of Low Radiation Pub Date : 2014-05-23 DOI:10.1504/IJLR.2014.060911

K. Mezhoud, Amina Sakly, H. B. Cheikh, M. Saidi, M. Edery

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引用次数: 1

Abstract

Radiology is an essential technology in medicine and is used for organ diagnosis, radio-tracing and radiotherapy. The risks for the radiobiology workers have not been assessed sufficiently because measuring instruments fail to detect very low doses. This paper presents an investigation on the potential risks for radiobiology workers, due to the occupational exposure to low doses of irradiation. In this respect we used plasma samples from 16 subjects who were supposed to receive very low dose of irradiation in a longer time period. We used chromosomal aberrations, means of oxidative stress measurement and combined proteomics and bioinformatics in order to elucidate risks of such exposure. We found significant chromosome aberrations in lymphocytes and the increase of oxidative stress biomarkers in plasma. In addition, the proteomic analysis shows differentially regulated proteins from which three were verified by ELISA tests. This proteomic analysis picks out some interesting proteins that may belong to biomarkers panel of radiation exposure.

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利用应激指标和蛋白质组学进行放射生物学工作者风险评估

放射学是医学中的一项基本技术，用于器官诊断、放射示踪和放射治疗。由于测量仪器无法检测到非常低的剂量，因此对放射生物学工作者的风险尚未进行充分评估。本文就低剂量辐照对放射生物学工作者的潜在危害进行了调查。在这方面，我们使用了16名受试者的血浆样本，他们应该在较长时间内接受非常低剂量的照射。我们使用染色体畸变、氧化应激测量手段以及结合蛋白质组学和生物信息学来阐明这种暴露的风险。我们发现淋巴细胞中有明显的染色体畸变，血浆中氧化应激生物标志物增加。此外，蛋白质组学分析显示了差异调节的蛋白质，其中三种通过ELISA测试验证。这种蛋白质组学分析挑选出一些有趣的蛋白质，可能属于辐射暴露的生物标志物面板。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis

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期刊介绍： The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.