Effect of Crude Extract of Clove Alcohol on the Biological Behaviour of PANC-1 Cells at 39?C

Abstract

Objective: To explore whether the crude extract of clove alcohol at 39 ˚C affects the proliferation, migration, invasion and apoptosis of pancreatic cancer cells. Methods: 1) CCK8 assay to detect the effect of crude extract of clove alcohol on the proliferation activity of pancreatic cancer cells at 39 ˚C ; 2) plate clone formation and soft agar cloning assay to detect the effect of crude extract of clove alcohol on the growth and proliferation of pancreatic cancer cells at 39 ˚C ; 3) scratch assay and Transwell assay to detect the effect of crude extract of clove alcohol on pancreatic cancer cells at 39 ˚C ; 4) Hoechst 33258 staining and flow cytometry to detect the effect of clove alcohol extract on cell morphological changes and apoptosis at 39 ˚C . Results: The results of CCK8 showed that the crude extract of clove alcohol could inhibit the growth and proliferation of PANC-1 cells, and the inhibitory effect was concentration-dependent, and the effect was more obvious in combination with 39 ˚C . The results of the scratch test showed that the crude extract of clove alcohol could inhibit the migration of PANC-1 cells, and the effect was more obvious in combination with 39°C, but there was no significant change between 37 ˚C and 39 ˚C control . The results of Hoechst33258 experiment showed that the crude extract of clove alcohol could cause the nucleus of PANC-1 cells to appear bright blue, apoptotic vesicles and nuclear fragmentation. The flow cytometry also verified that the crude extract of clove alcohol could induce apoptosis in PANC-1 cells with an increase in the number of apoptotic cells at 39 ˚C . Conclusion: The crude extract of clove alcohol can inhibit cell growth and proliferation, inhibit cell migration and invasion and induce apoptosis, and the effect is more obvious in combination with 39 ˚C , thus exerting anti-tumor effects from different mechanisms