Preparation of Cervical Ankang Gel and Preliminary Study on Pharmacodynamics

Abstract

Objective: To investigate the therapeutic effect of Cervical Ankang compound gel on cervicitis model rats. Methods: The extraction process of Cervical Ankang compound was optimized by orthogonal experimental design, and the prescription of Cervical Ankang compound Gel was optimized by uniform experimental design. A rat model of cervicitis was established, and the serum levels of IL-6, Hs-CRP, ultra-SOD and TNF- α were detected by kits. At the same time, HE staining was used to observe the pathological changes of rat cervix. Results: The optimal extraction process of Cervical Ankang compound was as follows, ethanol concentration 50%, extraction time 1.5 h, and extraction times 3 times. The optimal prescription of Cervical Ankang Gel was carbomer 5.0%, glycerol 16.4%, and sodium hydroxide 0.3 g. The results of the pharmacodynamics study showed that compared with the model group, the contents of IL-6 and TNF- α in the Cervical Ankang gel group decreased ( P < 0.05), the content of SOD increased ( P < 0.05), and the level of Hs-CRP was significantly decreased ( P < 0.01). The HE results showed that compared with the model group, the inflammatory cell infiltration in the cervical lesions of the rats in the Cervical Ankang gel group was significantly reduced. Conclusions: The successful preparation of the Cervical Ankang gel may be able to treat cervical inflammation by reducing the levels of IL-6, TNF- α and Hs-CRP in cervical lesions, increasing the levels of SOD, thereby affecting cytokines related to inflammatory response, and reducing the production of inflammatory exudates in cervical lesions.