Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

International journal of plant genomics Pub Date : 2015-12-28 DOI:10.1155/2015/261518

D. Politov, M. Belokon, Y. S. Belokon, T. Polyakova, A. Shatokhina, E. A. Mudrik, A. Azarova, M. Filippov, K. Shestibratov

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引用次数: 10

Abstract

Testing systems for molecular identification of micropropagated elite aspen (Populus tremula L.) genotypes were developed on the base on microsatellite (SSR) loci. Out of 33 tested microsatellite loci, 14 were selected due to sustainable PCR amplification and substantial variability in elite clones of aspen aimed for establishment of fast-rotated forest plantations. All eight tested clones had different multilocus genotypes. Among 114 trees from three reference native stands located near the established plantations, 80 haplotypes were identified while some repeated genotypes were attributed to natural clones which appeared as a result of sprouting. The selected set of SSR markers showed reliable individual identification with low probability of appearance of identical aspen genotypes (a minimum of 4.8 · 10−10 and 1 × 10−4 for unrelated and related individuals, resp.). Case studies demonstrating practical applications of the test system are described including analysis of clonal structure and levels of genetic diversity in three natural aspen stands growing in the regions where plantations made of elite clones were established.

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微卫星位点在水杨科白杨优良基因型分子鉴定、克隆性及遗传多样性分析中的应用

以微卫星(SSR)位点为基础，建立了微繁殖优良杨树(Populus tremula L.)基因型分子鉴定系统。在33个被测试的微卫星位点中，有14个是由于持续的PCR扩增和杨树精英无性系的大量变异而被选中的，目的是建立快速轮作的森林人工林。所有8个被测无性系均具有不同的多位点基因型。在已建立人工林附近的3个参考原生林分的114棵树中，鉴定出80个单倍型，而一些重复基因型归因于发芽后出现的自然无性系。所选择的SSR标记具有可靠的个体鉴定，出现相同杨树基因型的概率较低(无亲缘关系和亲缘关系的概率分别为4.8·10−10和1 × 10−4)。案例研究展示了测试系统的实际应用，包括对生长在建立优质无性系人工林地区的三种天然白杨林分的克隆结构和遗传多样性水平的分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of plant genomics

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