Spectroscopic and Electrochemical Studies on the Interaction of Epirubicin with Fish Sperm DNA

E-journal of Chemistry Pub Date : 2012-01-01 DOI:10.1155/2012/738678

R. Hajian, E. Ekhlasi, R. Daneshvar

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引用次数: 28

Abstract

The interactions between epirubicin (EPR) and double stranded DNA (ds-DNA) have been studied by UV-Vis spectrophotometry, cyclic voltammetry, fluorescence spectroscopy and viscometery. The apparent binding constant of epirubicin with DNA was found to be 3.8 × 105 mol-1 L and was studied at different temperatures. It indicated that the former method could be successfully applied to the determination of epirubicin. Also, the voltammetric behavior of EPR was investigated at glassy carbon electrode using cyclic voltammetry. Thermodynamic parameters including ∆H0, ∆G0 and ∆S0 were determined as -5.7×104, -3.13×104 J mol-1 and -87.96 J mol-1 K-1 respectively. One indication of the strong interaction between DNA and EP was increasing of viscosity. The diffusion coefficients of EP in the absence (D0)f and presence of ds-DNA (D0)b was calculated as 5.04×10-6 and 1.13×10-6 Cm2 s-1 respectively. According to the thermodynamic parameters, the main binding force could be judged. The experimental results revealed that EPR and ds-DNA had strong interactions. The mechanism of quenching belonged to static quenching and the main sort of binding force was intercalation.

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表柔比星与鱼精子DNA相互作用的光谱和电化学研究

采用紫外-可见分光光度法、循环伏安法、荧光光谱法和粘度法研究了表柔比星(EPR)与双链DNA (ds-DNA)的相互作用。表柔比星与DNA的表观结合常数为3.8 × 105 mol-1 L，并在不同温度下进行了研究。结果表明，该方法可用于表柔比星的测定。利用循环伏安法研究了EPR在玻碳电极上的伏安行为。∆H0、∆G0和∆S0的热力学参数分别为-5.7×104、-3.13×104 J mol-1和-87.96 J mol-1 K-1。DNA与EP之间强相互作用的一个迹象是粘度的增加。不存在(D0)f和存在ds-DNA (D0)b时EP的扩散系数分别计算为5.04×10-6和1.13×10-6 Cm2 s-1。根据热力学参数，可以判断主结合力。实验结果表明，EPR与ds-DNA具有较强的相互作用。淬火机理为静态淬火，结合力主要以插层作用为主。

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来源期刊

E-journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-

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审稿时长

3 months