Effects of chronic administration of pilocarpine on saliva secretion in streptozotocin-induced diabetic mice

Q4 Pharmacology, Toxicology and Pharmaceutics
Y. Nakagawa, H. Ishii, Takashi Saito, Takahiro Wakui, Taketomo Sato, Katsunori Ishibashii, M. Humphreys-Beher
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引用次数: 2

Abstract

Pilocarpine is a parasympathomimetic agonist and one of the most extensively studied salivary stimulants. Salivary hypofunction patients require long-term administration of secretagogues, but little is known about the effects of chronic administration of pilocarpine on saliva secretion. Hence, in the present study, we evaluated the effects of chronic administration of pilocarpine on saliva secretion in streptozotocin (STZ)-induced diabetic mice. Four weeks after induction of diabetes, saliva flow rate, protein concentration, and amylase activity were measured, by collecting saliva from the oral cavity over 30 min following an injection of pilocarpine. The effects of 7 days of pilocarpine administration were compared with the effects of a single injection. Saliva flow following a single intraperitoneal pilocarpine injection was significantly lower in the STZ diabetic mice than the control mice (buffer injection). With chronic administration of pilocarpine, the rate of saliva release increased significantly in the first 10-min period following injection, both in the diabetic and control mice, compared with the single pilocarpine injection results. The total amylase secretion over 30 min induced by a single injection of pilocarpine was not reduced in diabetic mice, despite a decrease in total salivary protein secretion. After chronic administration of pilocarpine, protein secretion increased both in the diabetic and control mice, compared with single injection levels. Chronic administration tended to increase total amylase activity in the control mice, but not the diabetic mice. The present study has clarified the effects of chronic administration of pilocarpine, and suggests that pilocarpine may be beneficial for patients with salivary gland dysfunction.
长期服用匹罗卡品对链脲佐菌素诱导的糖尿病小鼠唾液分泌的影响
匹罗卡品是一种拟副交感神经激动剂,也是研究最广泛的唾液兴奋剂之一。唾液功能减退患者需要长期服用促分泌剂,但长期服用匹罗卡品对唾液分泌的影响尚不清楚。因此,在本研究中,我们评估了长期服用匹罗卡品对链脲佐菌素(STZ)诱导的糖尿病小鼠唾液分泌的影响。在诱导糖尿病4周后,注射匹罗卡品后30分钟内,通过收集口腔唾液,测量唾液流速、蛋白质浓度和淀粉酶活性。将匹罗卡品给药7天的效果与单次注射的效果进行比较。单次腹腔注射匹罗卡品后,STZ糖尿病小鼠的唾液流量明显低于对照小鼠(缓冲注射)。与单次注射匹罗卡品相比,长期注射匹罗卡品后,糖尿病小鼠和对照组小鼠的唾液释放率在注射后的前10分钟内显著增加。在糖尿病小鼠中,单次注射匹罗卡品诱导的30分钟内的总淀粉酶分泌没有减少,尽管唾液总蛋白分泌减少。与单次注射相比,长期给药匹罗卡品后,糖尿病小鼠和对照组小鼠的蛋白质分泌均增加。慢性给药倾向于增加对照组小鼠的总淀粉酶活性,而不是糖尿病小鼠。本研究明确了长期服用匹罗卡品的影响,并提示匹罗卡品可能对唾液腺功能障碍患者有益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Oral Therapeutics and Pharmacology
Oral Therapeutics and Pharmacology Medicine-Pharmacology (medical)
CiteScore
0.10
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