ANTIOXIDANT EFFECT AND DNA PROTECTIVE EFFECT OF VARIOUS ENZYMATIC EXTRACTS FROM PERILLA FRUTESCENS VAR. CRISPA

Abstract

ABSTRACT

The antioxidant activity of enzymatic extracts from Perilla frutescens var. crispa was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and alkyl radical-scavenging activity by employing an electron spin resonance methodology. P. frutescens var. crispa was enzymatically hydrolyzed by eight carbohydrases (Dextrozyme, AMG, Promozyme, Maltogenase, Termamyl, Viscozyme, Celluclast, BAN [Novo Co., Novozyme Nordisk, Bagsvaerd, Denmark]) and nine proteases (Flavourzyme, Neutrase, Protamex, Alcalase, PP-trypsin [trypsin from porcine pancreas; Novo Co.], papain, pepsin, α-chymotrypsin, BP-trypsin [trypsin from bovine pancreas; Sigma Chemical Co., St. Louis, MO]). The DPPH radical-scavenging activities of Dextrozyme and Neutrase extracts from P. frutescens var. crispa were the highest and the IC₅₀ values were 60.12 and 112.81 µg/mL, respectively. All enzymatic extracts of P. frutescens var. crispa scavenged hydroxyl radical and the scavenging activity increased in a dose-dependent manner; the IC₅₀ values of Viscozyme and BP-trypsin, which showed the highest activity, were 0.78 and 0.26 mg/mL, respectively. In addition, the Termamyl and Protamex extracts of P. frutescens var. crispa showed the highest alkyl radical-scavenging activities, and the IC₅₀ values were 248.13 and 415.23 µg/mL, respectively. The Viscozyme and BP-trypsin extracts from P. frutescens var. crispa showed protective effect against H₂O₂-induced DNA damage. These results indicate that enzymatic extracts of P. frutescens var. crispa possess antioxidant activity.

PRACTICAL APPLICATIONS

Perilla frutescens var. crispa could be used to produce protein and carbohydrate extracts with antioxidative activeity. Many industrial commercial enzymes, such as Promozyme, Celluclast 1.5 L FG, Maltogenase L, Viscozyme L, Termamyl SC, Dextrozyme E, AMG 300 L, Protamex, Flavourzyme 500 MG, Neutrase 0.8 L, Puncreatic Trypsin and Alcalase 2.4 L, could also be used to attain the extracts processing the high antioxidative activity. The extracts can be used as natural antioxidants.