SIMPLE AND RAPID METHOD FOR DETECTING FOODBORNE SHIGELLA BY A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2009-12-02 DOI:10.1111/j.1745-4581.2009.00183.x

SULONG LI, XINBO ZHANG, DEGUO WANG, YANYUN KUANG, YIGANG XU

{"title":"SIMPLE AND RAPID METHOD FOR DETECTING FOODBORNE SHIGELLA BY A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION","authors":"SULONG LI, XINBO ZHANG, DEGUO WANG, YANYUN KUANG, YIGANG XU","doi":"10.1111/j.1745-4581.2009.00183.x","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n <h3> ABSTRACT</h3>\n \n <p> <i>A novel method was developed and evaluated for detecting</i> Shigella <i>by a loop-mediated isothermal amplification (LAMP) in this paper. Specific primers were specially designed and selected for recognizing seven distinct sequences of</i> ipaH <i>gene, which was performed only by</i> Shigella. <i>The sensitivity of the LAMP assay was 43 cfu/mL, approximately equal to that of real-time polymerase chain reaction assay. The LAMP assay developed in this study is specific, sensitive and suitable for the detection of foodborne</i> Shigella.</p>\n </section>\n \n <section>\n \n <h3> PRACTICAL APPLICATION</h3>\n \n <p>The LAMP method reported here provided a powerful tool for detection of foodborne <i>Shigella</i> due to its specificity, sensitivity and rapidity.</p>\n </section>\n </div>","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"17 4","pages":"465-475"},"PeriodicalIF":0.0000,"publicationDate":"2009-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2009.00183.x","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Rapid Methods and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.2009.00183.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 12

Abstract

ABSTRACT

A novel method was developed and evaluated for detecting Shigella by a loop-mediated isothermal amplification (LAMP) in this paper. Specific primers were specially designed and selected for recognizing seven distinct sequences of ipaH gene, which was performed only by Shigella. The sensitivity of the LAMP assay was 43 cfu/mL, approximately equal to that of real-time polymerase chain reaction assay. The LAMP assay developed in this study is specific, sensitive and suitable for the detection of foodborne Shigella.

PRACTICAL APPLICATION

The LAMP method reported here provided a powerful tool for detection of foodborne Shigella due to its specificity, sensitivity and rapidity.

查看原文本刊更多论文

环介导等温扩增检测食源性志贺氏菌的简单快速方法

本文建立了一种新的检测志贺氏菌的环介导等温扩增(LAMP)方法。针对7个不同的ipaH基因序列，专门设计和选择了特异性引物，仅由志贺氏菌进行识别。LAMP法的灵敏度为43 cfu/mL，与实时聚合酶链反应法的灵敏度相近。本研究建立的LAMP检测方法特异性强，灵敏度高，适用于食源性志贺氏菌的检测。LAMP方法具有特异性、敏感性和快速性，为食源性志贺氏菌的检测提供了有力的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Rapid Methods and Automation in Microbiology 生物-生物工程与应用微生物

自引率

0.00%

发文量