AN INNOVATIVE MICROPLATE ASSAY TO FACILITATE THE DETECTION OF ANTIMICROBIAL ACTIVITY IN PLANT EXTRACTS

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2009-12-02 DOI:10.1111/j.1745-4581.2009.00187.x

Y. SULTANBAWA, A. CUSACK, M. CURRIE, C. DAVIS

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引用次数: 30

Abstract

ABSTRACT

A microplate assay was modified for the detection of antimicrobial activity in plant extracts. The aim was to develop an in vitro assay that could rapidly screen plant extracts to provide quantitative data on inhibition of microbial growth. A spectrophotometric assay using a microplate with serial dilutions of the plant extract and the bacteria was developed. Two bacteria, Staphylococcus aureus and Escherichia coli, were used for this study. Essential oils, oregano (Origanum vulgare) and lemon myrtle (Backhousia citriodora), and three active components carvacrol, thymol and citral were evaluated. The reproducibility of the assay was high, with correlation coefficients (r²) of the replicates of lemon myrtle and oregano with S. aureus and E. coli between 0.9321 and 0.9816. Similarly, r² values for carvacrol, thymol and citral were between 0.8455 and 0.9814. This assay could also be used to measure antimicrobial activity in plant extracts which vary in pH and color.

PRACTICAL APPLICATIONS

This research could be used for the quantitative determination of antimicrobial activity in plant extracts. As inhibition of growth is expressed as a percentage from 0 to 100, this method could also be used to study synergies within plant extracts to enhance antimicrobial activity for future studies. When plant extracts are used in food as natural preservatives, the challenge is to use a concentration that does not interfere with the flavor of the food product. In general, the amount of plant extract required to extend the storage life of food is greater than that required to produce an acceptable flavor. The advantage of knowing the percent inhibition relates to the effect of synergies where the inhibitions less than 100% can be enhanced with other antimicrobial plant extracts, thereby reducing the amount of plant extract required.

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一个创新的微孔板试验，以方便检测抗微生物活性的植物提取物

摘要:本文对检测植物提取物抑菌活性的微孔板法进行了改进。目的是建立一种能够快速筛选植物提取物的体外测定方法，以提供抑制微生物生长的定量数据。采用微孔板对植物提取物和细菌进行连续稀释，建立了分光光度测定法。本研究采用了金黄色葡萄球菌和大肠杆菌两种细菌。对精油、牛至(Origanum vulgare)和柠檬桃金娘(Backhousia citriodora)以及香芹酚、百里香酚和柠檬醛三种有效成分进行了评价。该方法重复性高，柠檬桃金子和牛至与金黄色葡萄球菌和大肠杆菌的相关系数(r2)在0.9321 ~ 0.9816之间。同样，香芹酚、百里酚和柠檬醛的r2值在0.8455 ~ 0.9814之间。该试验也可用于测定不同pH值和颜色的植物提取物的抗菌活性。本研究可用于植物提取物抗菌活性的定量测定。由于对生长的抑制作用以0 ~ 100的百分比表示，因此该方法也可用于研究植物提取物内部的协同作用，以增强抗菌活性，为未来的研究提供依据。当食品中使用植物提取物作为天然防腐剂时，挑战在于使用不影响食品风味的浓度。一般来说，延长食品储存寿命所需的植物提取物量大于产生可接受风味所需的植物提取物量。知道抑制百分比的优势在于协同作用的效果，其中低于100%的抑制可以与其他抗菌植物提取物一起增强，从而减少所需的植物提取物量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Rapid Methods and Automation in Microbiology 生物-生物工程与应用微生物

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