Polymorphisms within the HLA-DR4 haplotypes. Various DQ subtypes detected with monoclonal antibodies.

Abstract

Polymorphisms among HLA class II molecules expressed by cells with different HLA-DR4 haplotypes were analysed biochemically (isoelectrofocussing and 2D gels), cellularly (HLA-Dw) and serologically (monoclonal antibodies). The results confirm the correlation which exists between HLA-D specificity and DR beta chain isoelectric point polymorphism. Furthermore, a biochemical polymorphism was observed among DQw3 molecules. No correlation was found with HLA-Dw types. On the other hand a correlation was found between DQ-polymorphism and TA10 and 2B3 specificities defined by monoclonal antibodies. The comparison of different methods defining polymorphisms of HLA class II molecules will be discussed.