The zinc-finger transcription factor LSL-1 is a major regulator of the germline transcriptional program in Caenorhabditis elegans.

IF 3.3 3区 生物学
Genetics Pub Date : 2022-05-05 DOI:10.1093/genetics/iyac039
David Rodriguez-Crespo, Magali Nanchen, Shweta Rajopadhye, Chantal Wicky
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引用次数: 0

Abstract

Specific gene transcriptional programs are required to ensure the proper proliferation and differentiation processes underlying the production of specialized cells during development. Gene activity is mainly regulated by the concerted action of transcription factors and chromatin proteins. In the nematode Caenorhabditis elegans, mechanisms that silence improper transcriptional programs in germline and somatic cells have been well studied, however, how are tissue-specific sets of genes turned on is less known. LSL-1 is herein defined as a novel crucial transcriptional regulator of germline genes in C. elegans. LSL-1 is first detected in the P4 blastomere and remains present at all stages of germline development, from primordial germ cell proliferation to the end of meiotic prophase. lsl-1 loss-of-function mutants exhibit many defects including meiotic prophase progression delay, a high level of germline apoptosis, and production of almost no functional gametes. Transcriptomic analysis and ChIP-seq data show that LSL-1 binds to promoters and acts as a transcriptional activator of germline genes involved in various processes, including homologous chromosome pairing, recombination, and genome stability. Furthermore, we show that LSL-1 functions by antagonizing the action of the heterochromatin proteins HPL-2/HP1 and LET-418/Mi2 known to be involved in the repression of germline genes in somatic cells. Based on our results, we propose LSL-1 to be a major regulator of the germline transcriptional program during development.

锌指转录因子 LSL-1 是秀丽隐杆线虫种系转录程序的主要调节因子。
在发育过程中,需要特定的基因转录程序来确保特化细胞的正常增殖和分化过程。基因活动主要受转录因子和染色质蛋白的协同作用调控。在线虫秀丽隐杆线虫(Caenorhabditis elegans)中,人们对抑制生殖细胞和体细胞中不适当转录程序的机制进行了深入研究,但对组织特异性基因如何开启却知之甚少。本文将 LSL-1 定义为 elegans 生殖系基因的一种新型关键转录调节因子。lsl-1功能缺失突变体表现出许多缺陷,包括减数分裂前期进展延迟、高水平的生殖细胞凋亡以及几乎不产生功能配子。转录组分析和 ChIP-seq 数据显示,LSL-1 与启动子结合,是生殖系基因的转录激活因子,参与同源染色体配对、重组和基因组稳定性等多个过程。此外,我们还发现,LSL-1 通过拮抗异染色质蛋白 HPL-2/HP1 和 LET-418/Mi2 的作用而发挥作用,已知这两种蛋白参与了体细胞中生殖系基因的抑制作用。基于我们的研究结果,我们认为 LSL-1 是发育过程中生殖系转录程序的主要调节因子。
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来源期刊
Genetics
Genetics 生物-遗传学
CiteScore
6.20
自引率
6.10%
发文量
177
期刊介绍: GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.
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