The potential effect of vanadium compounds on glucose-6-phosphatase

Abstract

glucose-6-phosphatase from bovine liver microsomes using differential centrifugation, and then the enzyme was assayed in the presence and absence of vanadium compounds. The study found that vanadyl compounds inhibit glucose-6-phosphatase, as mean specific enzyme activity was calculated which showed that VOSO 4 at a concentration of 48 µM inhibited glucose6-phosphatase activity by 36.9% (P < 0.0003) and Vace at a concentration of 200 µM inhibited glucosse-6-phosphatase activity by 50% (P < 0.0001) similar to findings in the previous research. However, this study also found using Lineweaver–Burk plot analysis that VOSO4 is a competitive inhibitor of glucose-6-phophatase and Vace is a mixed: non-competitive and uncompetitive inhibitors of glucose-6-phosphatase. The mechanism of inhibitory action of these vanadyl compounds had not been reported previously. The difference in the concentration of inhibitor required may be due to the type of inhibition. This supports the hypothesis to some extent as the results were found to be statistically significant; however, further data will be required to clarify these findings.