Storage stabilization of microbes for biotechnology

Abstract

https://pubmed.ncbi.nlm.nih.gov/25773973/

In culture collections, microbes are typically stored after freeze-drying. This study examined microorganisms known to be recalcitrant to storage and examined drying conditions that would lead to the best outcomes.

https://www.thermofisher.com/us/en/home/industrial/microbiology/microbiology-learning-center/storing-bacterial-samples-optimal-viability.html

This commercial website provides a good overview of different bacterial storage methods, the temperatures they require and the effects on bacteria generally.

https://www.nature.com/articles/s41598-019-38588-6

Increasingly, algal storage is becoming industrially relevant. This study used Chlorella vulgaris as an example and showed that ultra-low temperature and nitrogen control were important factors in maintaining viability.

https://www.intechopen.com/books/probiotics/different-methods-of-probiotics-stabilization

This report describes a wide range of methods for preparation and storage of probiotic bacterial starter cultures.

https://link.springer.com/article/10.1007/s00253-017-8706-6

This study examined Arthrobacter chlorophenolicus A6 that biodegrades chlorophenols. They examined methods like air-drying in media such as vermiculite, to store bacteria for use in bioremediation.

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0227486

Given the increasing interest in metagenomic studies of gut microbiomes, accurate and reproducible comparative data often requires maintaining microbial viability while storing stool samples. This study examined storage conditions in this context.

https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.1046

This study describes the use of a guanidine thiocyanate based medium to stabilize microbial DNA in gut microbiome sample for shipping, storage and later sequencing.

https://link.springer.com/article/10.1007/s00294-019-01036-z

Trehalose has been known to prevent cellular protein damage by acting as a chemical chaperone, but it might also diminish protein acetylation and glycation.

https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.12880

This review article discusses methods for maintaining high viability in microbial inoculant cultures for use in agriculture.

https://patents.google.com/patent/US8011132B2/en

Numerous genera of bacteria have been shown to provide benefits to plant growth and those include: Rhizobium, Bradyrhizobium, Bacillus, Azotobacter and Azospirillum species. This patent deals with the storage of these types of bacteria in liquid inoculants on seeds.

http://www.esalq.usp.br/lepse/imgs/conteudo_thumb/Life-and-death-of-dried-prokaryotes.pdf

This classic paper focuses on the underlying molecular mechanisms of desiccation resistance and sensitivity in prokaryotes.

https://www.biorxiv.org/content/10.1101/2021.02.06.430066v1.full

This preprint deals with desiccation tolerant bacteria in cold adapted dry environments where water is in a solid state during almost the entire year.