Alleviation of cadmium toxicity in common bean (Phaseolus vulgaris L.) plants by the exogenous application of salicylic acid

Abstract

Summary A two-season pot experiment was conducted on Phaseolus vulgaris L. plants to evaluate their response to 1.0 mM salicylic acid (SA) in a growing medium contaminated with 0.25 or 0.50 mM Cd2+ ions. Plants were sampled for growth measurements and chemical analyses 45 d after sowing, and to measure the yield of beans at the end of each experiment. Exposing plants to either concentration of Cd2+ ions resulted in significant declines in growth, pigment concentrations, relative water content, and nutrient concentrations, and in chlorophyll fluorescence (Fv/Fm) and the performance index (PI) of photosynthesis. However, 1.0 mM SA mitigated Cd2+ ion stress and significantly improved each of these parameters. Both Cd2+ ion treatments increased proline and Cd2+ ion concentrations, electrolyte leakage, and lipid peroxidation (measured as malondialdehyde concentration). However, 1.0 mM SA attenuated the adverse effects of Cd2+ ions on these characteristics. Cd2+-induced increases in the activities of several key anti-oxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase were reduced following the exogenous application of 1.0 mM SA.These results confirm that the application of 1.0 mM SA could be used to reduce the adverse effects of Cd2+ ion toxicity in bean plants.