Somatic embryogenesis and plant regeneration in black pepper (Piper nigrum L.): I. Direct somatic embryogenesis from tissues of germinating seeds and ontogeny of somatic embryos

IF 1.7 4区农林科学 Q2 HORTICULTURE

Journal of Horticultural Science & Biotechnology Pub Date : 2003-01-01 DOI:10.1080/14620316.2003.11511641

R. Ramakrishnan Nair, S. Dutta Gupta

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引用次数: 23

Abstract

Summary A protocol was developed for induction, maturation and germination of somatic embryos from the tissues of germinating seeds of black pepper (Piper nigrum L.). Explants were cultured on growth regulator – free solid SH medium maintained in the dark. The first somatic embryos developing directly from the explant tissue were noticed after 60 d of culture. Somatic embryos originated from a ring-like tissue on the micropylar region of the seeds. Sucrose concentration of the medium was found to be crucial for the induction of somatic embryos, and 30 g l–1 was found to be the optimum. Maturation and germination of somatic embryos were achieved on the same medium. Suspension culture enhanced the process of maturation and germination. Regenerated plants were established in soil. Histology confirmed the ontogeny and each stage of development. Growth regulators were found to inhibit the induction of somatic embryogenesis. Cytological analysis of the regenerated plants revealed the normal chromosome number of 2n=52.

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黑胡椒的体胚发生与植株再生:1 .萌发种子组织直接体胚发生与体胚的个体发生

研究了黑胡椒萌发种子组织体胚的诱导、成熟和萌发过程。外植体在无生长调节剂的固体SH培养基上培养，并保持在黑暗中。培养60 d后，第一批体胚直接由外植体组织发育而来。体细胞胚起源于种子微孔区域的环状组织。培养基中蔗糖浓度对体胚的诱导至关重要，以30g - 1为最佳。体细胞胚在同一培养基上成熟和萌发。悬浮培养促进了种子的成熟和萌发。在土壤中建立了再生植物。组织学证实了个体发生和发育的各个阶段。生长调节剂可抑制体细胞胚胎发生的诱导。再生植株细胞学分析显示正常染色体数为2n=52。

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来源期刊

Journal of Horticultural Science & Biotechnology 农林科学-园艺

CiteScore

3.90

自引率

5.30%

发文量

审稿时长

3 months

期刊介绍： The Journal of Horticultural Science and Biotechnology is an international, peer-reviewed journal, which publishes original research contributions into the production, improvement and utilisation of horticultural crops. It aims to provide scientific knowledge of interest to those engaged in scientific research and the practice of horticulture. The scope of the journal includes studies on fruit and other perennial crops, vegetables and ornamentals grown in temperate or tropical regions and their use in commercial, amenity or urban horticulture. Papers, including reviews, that give new insights into plant and crop growth, yield, quality and response to the environment, are welcome, including those arising from technological innovation and developments in crop genome sequencing and other biotechnological advances.