The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Q1 Biochemistry, Genetics and Molecular Biology

Frontiers in Life Science Pub Date : 2016-01-02 DOI:10.1080/21553769.2015.1077477

M. Ghiasi, N. Kalhor, Reza Tabatabaei Qomi, Mohsen Sheykhhasan

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引用次数: 26

Abstract

ABSTRACT This study presents a comparative assessment of adipose-derived stem cell (ADSCs) proliferation rates and their viability on five different scaffolds. Five different biomaterial scaffolds were prepared: alginate, poly lactic-co-glycolic acid, fibrin glue, inactive platelet-rich plasma, and active platelet-rich plasma (APRP). Stem cells were isolated from human adipose tissue. Flow cytometry analysis was performed. Specifically, adipogenesis/osteogenesis/chondrogenesis-associated genes expression was analyzed by real-time polymerase chain reaction. These cells were seeded in the prepared scaffolds. After 14 days, the proliferation and viability of MSCs were evaluated using an MTT assay. Also, stemness genes expression was analyzed with the reverse transcriptasepolymerase chain reaction (RT-PCR) method. In addition, the DNA content assay was also performed. The obtained results showed a significant difference between cell proliferation and viability of different scaffolds. APRP and alginate were shown to be the most and least suitable scaffolds in terms of enhancing cell proliferation and maintaining cell viability respectively (p < .05). RT-PCR reactions demonstrated the expression of the various stemness-related markers (Nanog, Octamer4A, and Sox2) when ADSC cells were grown separately on the five different scaffolds. Our study indicates that compared with the scaffolds, APRP could be the best scaffold for support of ADSC proliferation.

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合成支架和天然支架对脂肪干细胞活力和增殖的影响

摘要:本研究比较了脂肪源性干细胞(ADSCs)在五种不同支架上的增殖率及其生存能力。制备海藻酸盐、聚乳酸-羟基乙酸、纤维蛋白胶、无活性富血小板血浆和活性富血小板血浆(APRP)五种不同的生物材料支架。从人脂肪组织中分离出干细胞。流式细胞术分析。具体来说，通过实时聚合酶链反应分析脂肪生成/成骨/软骨生成相关基因的表达。将这些细胞植入制备的支架中。14天后，采用MTT法评估MSCs的增殖和活力。用逆转录聚合酶链式反应(RT-PCR)方法分析干性基因的表达。此外，还进行了DNA含量测定。结果表明，不同支架的细胞增殖和活力存在显著差异。在促进细胞增殖和维持细胞活力方面，APRP和海藻酸盐分别是最适合和最不适合的支架材料(p < 0.05)。RT-PCR反应显示，当ADSC细胞分别在5种不同的支架上生长时，多种干细胞相关标志物(Nanog、Octamer4A和Sox2)均有表达。我们的研究表明，与支架相比，APRP可能是支持ADSC增殖的最佳支架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Life Science MULTIDISCIPLINARY SCIENCES-

CiteScore

5.50

自引率

0.00%

发文量

期刊介绍： Frontiers in Life Science publishes high quality and innovative research at the frontier of biology with an emphasis on interdisciplinary research. We particularly encourage manuscripts that lie at the interface of the life sciences and either the more quantitative sciences (including chemistry, physics, mathematics, and informatics) or the social sciences (philosophy, anthropology, sociology and epistemology). We believe that these various disciplines can all contribute to biological research and provide original insights to the most recurrent questions.