Research Note: Quantification of Increase in Hepatic CYP1A1 mRNA by Real-Time RT-PCR after Exposure of the European Eel (Anguilla anguilla) to a Diesel Oil Water Soluble Fraction

Environmental bioindicators Pub Date : 2007-04-10 DOI:10.1080/15555270701263002

É. Aubry, C. Cagnon, Y. Lalanne, C. Mouchès

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引用次数: 1

Abstract

Editors' note: Research Notes is a new section now being added to the EBI format. Its purpose is to provide a forum for the publication of short research communications that provide important information but, due to their restricted focus, are not formatted in the manner of full research papers. All papers published under this heading will be peer reviewed. In order to follow the effects of pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorobenzenes (PCBs), and dioxins upon the European eel, we have developed an assay that quantifies hepatic cytochrome P4501A1 (CYP1A1) mRNA levels using real-time reverse transcription polymerase chain reaction (real-time RT-PCR). In this study, European eels were exposed to a diesel water soluble fraction (DWSF) in order to mimic environmental exposure to a mixture of organic pollutants. CYP1A1 mRNA levels were measured after 3, 6, 12, 24, 48, 72 and 156 h of exposure. The 7-ethoxyresorufin O-deethylase (EROD) activity was measured in parallel to confir...

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研究说明:将欧洲鳗鲡(Anguilla Anguilla)暴露于柴油水溶性馏分后，用实时荧光定量pcr方法定量肝脏CYP1A1 mRNA的增加

编者注:研究笔记是一个新的部分，现在被添加到EBI格式。它的目的是为提供重要信息的简短研究通讯的出版提供一个论坛，但由于它们的重点有限，不像完整的研究论文那样格式化。所有在此标题下发表的论文都将经过同行评审。为了跟踪多环芳烃(PAHs)、多氯苯(PCBs)和二恶英等污染物对欧洲鳗鱼的影响，我们开发了一种利用实时逆转录聚合酶链反应(real-time RT-PCR)定量肝细胞色素P4501A1 (CYP1A1) mRNA水平的测定方法。在这项研究中，欧洲鳗鱼暴露于柴油水溶性组分(DWSF)中，以模拟环境暴露于有机污染物混合物中。在暴露3、6、12、24、48、72和156 h后检测CYP1A1 mRNA水平。平行测定了7-乙氧基间苯二酚o -去乙基酶(EROD)活性，以证实其有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Environmental bioindicators

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