Microfluidic high-throughput single-cell mechanotyping: Devices and applications

IF 3.5 3区工程技术 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nami Jishu yu Jingmi Gongcheng/Nanotechnology and Precision Engineering Pub Date : 1900-01-01 DOI:10.1063/10.0006042

G. Choi, Zifan Tang, W. Guan

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引用次数: 7

Abstract

The mechanical behavior of individual cells plays an important role in regulating various biological activities at the molecular and cellular levels. It can serve as a promising label-free marker of cells’ physiological states. In the past two decades, several techniques have been developed for understanding correlations between cellular mechanical changes and human diseases. However, numerous technical challenges remain with regard to realizing high-throughput, robust, and easy-to-perform measurements of single-cell mechanical properties. In this paper, we review the emerging tools for single-cell mechanical characterization that are provided by microfluidic technology. Different techniques are benchmarked by considering their advantages and limitations. Finally, the potential applications of microfluidic techniques based on cellular mechanical properties are discussed. © 2021 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). https://doi.org/10.1063/10.0006042

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微流体高通量单细胞机械分型:设备和应用

单个细胞的力学行为在分子和细胞水平上调控各种生物活动中起着重要作用。它可以作为细胞生理状态的无标记标记物。在过去的二十年中，已经开发了几种技术来了解细胞力学变化与人类疾病之间的相关性。然而，在实现高通量、鲁棒性和易于执行的单细胞机械性能测量方面，仍然存在许多技术挑战。在本文中，我们回顾了微流体技术提供的单细胞力学表征的新兴工具。通过考虑不同技术的优点和局限性，对它们进行基准测试。最后，讨论了基于细胞力学特性的微流控技术的潜在应用前景。©2021作者。除另有说明外，所有文章内容均遵循知识共享署名(CC BY)许可协议(http://creativecommons.org/licenses/by/4.0/)。https://doi.org/10.1063/10.0006042

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