Hepatic and skeletal muscle mitochondrial toxicity of chitosan oligosaccharides of normal and diabetic rats

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2016-10-28 DOI:10.1080/15376516.2016.1222643

J. Teodoro, A. P. Gomes, A. T. Varela, F. V. Duarte, A. Rolo, C. Palmeira

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引用次数: 10

Abstract

Abstract Diabetes and associated conditions are now considered a worldwide epidemic, with increasing costs and burdens with no cure yet developed. The chitin-derived glucosamine biopolymer chitosan has shown promising results when supplied to diabetic patients. However, no study has investigated the possible toxic side effects of chitosan treatments, in particular when regarding the most important bioenergetic organelle, mitochondria. As such, we aimed to understand if supplementation of chitosan to the diet of normal and diabetic rats could compromise mitochondrial function on two of the major organs involved in diabetes, obesity, and metabolic regulation, the liver and skeletal muscle. We supplemented the drinking water of normal Wistar and diabetic Goto–Kakizaki rats with 0.5% chitosan for 6 weeks. We show here that, in terms of hepatic bioenergetics, chitosan was relatively inert and had no major side effects. However, regarding skeletal muscle bioenergetics, chitosan significantly affected various bioenergetic parameters. As such, we conclude that chitosan, at the tested doses, is relatively safe for treatment of diabetic situations. Nonetheless, the potential for adverse toxicological side effects appears to be present, which might be relevant if higher doses are utilized.

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壳寡糖对正常和糖尿病大鼠肝脏和骨骼肌线粒体毒性的影响

糖尿病及其相关疾病现在被认为是一种世界性的流行病，其成本和负担不断增加，但尚未开发出治疗方法。甲壳素衍生的氨基葡萄糖生物聚合物壳聚糖在治疗糖尿病方面显示出良好的效果。然而，没有研究调查壳聚糖处理可能的毒副作用，特别是当涉及最重要的生物能量细胞器线粒体时。因此，我们的目的是了解在正常和糖尿病大鼠的饮食中添加壳聚糖是否会损害与糖尿病、肥胖和代谢调节有关的两个主要器官——肝脏和骨骼肌的线粒体功能。在正常Wistar大鼠和糖尿病后崎大鼠的饮水中添加0.5%壳聚糖，持续6周。我们在这里表明，在肝脏生物能量学方面，壳聚糖是相对惰性的，没有主要的副作用。然而，在骨骼肌生物能量学方面，壳聚糖对各种生物能量参数有显著影响。因此，我们得出结论，在测试剂量下，壳聚糖对糖尿病的治疗是相对安全的。尽管如此，潜在的毒理学副作用似乎是存在的，如果使用更高的剂量，这可能是相关的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.