Alleviation of bone markers in rats induced nano-zinc oxide by qurecetin and α-lipolic acid

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2016-10-27 DOI:10.1080/15376516.2016.1236424

Hala M Abdelkarem, Laila M. Fadda, Omyma R Kaml

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引用次数: 10

Abstract

Abstract The purpose of this study was to evaluate the potential protective effect of qurecetin (Qur) and α-lipolic acid (ALA) to modulate the perturbation of bone turnover which is induced by nano-zinc oxide (n-ZnO). Rats were fasted overnight and randomly divided into two groups: G1, normal healthy animals and the other rats were administered zinc oxide nanoparticles orally by guava in a dose of 600 mg/kg body weight/d for 5 sequential days in Wistar albino male rats. N-ZnO-exposed animals were randomly sub-divided into three groups: G2, n-ZnO-exposed animals; G3, n-ZnO-exposed animals co-treated with Qur (200 mg/kg daily); and G4, n-ZnO-exposed animals co-treated with ALA (200 mg/kg). Qur and ALA were administered orally by guava daily for three sequential weeks from the beginning of the experiment. The results revealed a significant reduction of nitiric oxide (NO) and serum level and comet assay in n-ZnO exposure rats after treatment of Qur and ALA. It was found the alteration of pro-inflammatory markers (tumor necrosis factor alpha; TNF-α, interleukin-6; IL-6 and C-reactive protein; CRP), bone alkaline phosphatase (B-ALP, bone formation marker), and C-terminal peptide type I collagen (CTx, bone resorption marker) levels compared with the normal group. Co-administration of Qur and ALA in n-ZnO-exposed rats significantly alleviated the mentioned alterations of biochemical parameters. These results suggest that Qur and ALA as antioxidant agents may be a candidate for preventive and treatment applications of impaired bone markers induced bone loss caused by nano-particles of metal oxide.

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槲皮素和α-脂酸对纳米氧化锌诱导大鼠骨标志物的缓解作用

摘要本研究旨在探讨槲皮素(Qur)和α-脂酸(ALA)对纳米氧化锌(n-ZnO)诱导的骨转换紊乱的潜在保护作用。禁食1夜，随机分为两组:G1组，正常健康大鼠，其余Wistar白化雄性大鼠口服番石榴氧化锌纳米颗粒，剂量为600 mg/kg体重/d，连续5 d。n- zno暴露动物随机分为3组:G2组，n- zno暴露动物;G3、n- zno暴露动物与Qur共处理(200mg /kg每日);G4、n- zno暴露动物与ALA共处理(200 mg/kg)。从实验开始，每天通过番石榴口服Qur和ALA，连续三周。结果显示，在n-ZnO暴露大鼠中，quur和ALA处理后，一氧化氮(NO)和血清水平以及彗星测定均显著降低。发现促炎标志物(肿瘤坏死因子α;肿瘤坏死因子-α,白细胞介素- 6;IL-6和c反应蛋白;CRP)、骨碱性磷酸酶(B-ALP，骨形成标志物)、c端肽I型胶原蛋白(CTx，骨吸收标志物)水平与正常组比较。n- zno暴露大鼠同时给药Qur和ALA可显著减轻上述生化参数的改变。这些结果表明，Qur和ALA作为抗氧化剂可能是预防和治疗纳米金属氧化物颗粒引起的骨标志物损伤引起的骨质流失的候选药物。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.