Does age matter? Comparison of neurobehavioral effects of paraquat exposure on postnatal and adult C57BL/6 mice

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2016-11-06 DOI:10.1080/15376516.2016.1223241

D. Lou, Qiaochu Wang, Min Huang, Zhijun Zhou

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引用次数: 12

Abstract

Abstract Epidemiological studies have revealed that environmentally relevant low levels of paraquat (PQ) exposure is listed on the etiology of neurological disorders such as Parkinson’s disease (PD). The behavioral effects of PQ are of current interest, especially when exposure occurs in the period of early stage of life. To characterize whether and how age affects neurobehavioral performances of mice after PQ exposure, 21 days postnatal (PN21) and adult male C57BL/6 mice were daily administrated by oral gavage with 0 mg/kg (saline, control), 5 mg/kg or 10 mg/kg of PQ for 28 consecutive days. Survival rate and body weight were analyzed. Subsequently, mice were subjected to Morris water maze tests (MWM). The results showed that mice exposed to PQ had significantly longer latencies than those in the control group, with a dose-dependent manner. Furthermore, PN21 mice tended to have longer latencies than adult mice in the same dose group. Our data suggested that PQ exposure induced significant learning and memory impairment and more severely in PN21 mice when compared with adult mice.

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年龄有关系吗?百草枯暴露对产后和成年C57BL/6小鼠神经行为影响的比较

流行病学研究表明，环境相关的低水平百草枯(PQ)暴露是帕金森病(PD)等神经疾病的病因。PQ的行为影响是当前的兴趣，特别是当暴露在生命的早期阶段。为了研究年龄是否以及如何影响PQ暴露后小鼠的神经行为表现，在出生后21天(PN21)，成年雄性C57BL/6小鼠每天灌胃0 mg/kg(生理盐水，对照组)、5 mg/kg或10 mg/kg的PQ，连续28天。分析成活率和体重。随后，小鼠进行Morris水迷宫实验(MWM)。结果显示，暴露于PQ的小鼠潜伏期明显长于对照组，且呈剂量依赖性。此外，在相同剂量组，PN21小鼠比成年小鼠具有更长的潜伏期。我们的数据表明，与成年小鼠相比，PQ暴露会导致PN21小鼠显著的学习和记忆障碍，并且更严重。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.