Molecularly imprinted polymer preparations for selective detection of C-reactive protein: Thermodynamic and kinetic studies

IF 2.702 Q1 Materials Science
?i?dem ?ter, Abdulqader Tawfeeq
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引用次数: 0

Abstract

C-reactive protein (CRP) is a member of the pentraxin protein group. CRP is considered an acute-phase protein produced by the liver during inflammation in various diseases limited to pathogenic infections. It is very important that serum CRP concentration can be measured quickly, reliably and easily. Therefore, a three-dimensional crosslinked molecularly imprinted polymer (MIP) with selective recognition sites for CRP was synthesized (CRP-MIP) and characterization analyzes (scanning electron microscopy, Fourier transform infrared, and thermogravimetric analysis) were performed. The binding abilities of the synthesized polymers by adsorption of CRP in aqueous solution were evaluated in detail and compared with the abilities of an unprinted polymer (CRP-NIP) used as a reference. It was found that the MIP prepared by the printing effect selectively adsorbed the template molecule CRP. For this effect, the selectivity of MIP toward CRP and various positive acute-phase reactants such as α1-antitrypsin and α1-acid glycoprotein was evaluated and high selectivity toward CRP was obtained. CRP-MIP was used to remove CRP from crude human serum, and the recovery was up to 91%. Adsorption process of CRP from aqueous solutions on polymeric adsorbents; equilibrium was evaluated in terms of kinetic and thermodynamic conditions and the necessary parameters to describe the process were calculated under these conditions. Adsorption data: the pseudo-first order kinetic model, the pseudo-second order kinetic model, the Elovich kinetic model and the intraparticle diffusion model were studied and the thermodynamic parameters ΔG°, ΔH°, and ΔS° were calculated.

Abstract Image

选择性检测c反应蛋白的分子印迹聚合物制备:热力学和动力学研究
c反应蛋白(CRP)是戊素蛋白组的一员。CRP被认为是一种急性期蛋白,由肝脏在各种疾病的炎症过程中产生,仅限于致病性感染。快速、可靠、简便地测定血清CRP浓度具有重要意义。因此,合成了一种具有CRP选择性识别位点的三维交联分子印迹聚合物(CRP-MIP),并进行了表征分析(扫描电镜、傅里叶变换红外和热重分析)。详细评价了通过吸附CRP合成的聚合物在水溶液中的结合能力,并与未打印的聚合物(CRP- nip)的结合能力进行了比较。研究发现,利用打印效应制备的MIP可选择性吸附模板分子CRP。为此,MIP对CRP和α1-抗胰蛋白酶、α1-酸性糖蛋白等多种急性期阳性反应物的选择性进行了评价,获得了对CRP的高选择性。采用CRP- mip法去除人粗血清中的CRP,回收率达91%。高分子吸附剂对CRP水溶液的吸附过程在动力学和热力学条件下评估了平衡状态,并在这些条件下计算了描述该过程的必要参数。吸附数据:研究了拟一级动力学模型、拟二级动力学模型、Elovich动力学模型和颗粒内扩散模型,并计算了热力学参数ΔG°、ΔH°和ΔS°。
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来源期刊
CiteScore
5.20
自引率
0.00%
发文量
0
审稿时长
1.8 months
期刊介绍: Part A: Polymer Chemistry is devoted to studies in fundamental organic polymer chemistry and physical organic chemistry. This includes all related topics (such as organic, bioorganic, bioinorganic and biological chemistry of monomers, polymers, oligomers and model compounds, inorganic and organometallic chemistry for catalysts, mechanistic studies, supramolecular chemistry aspects relevant to polymer...
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