Fundamental insights in early-stage inclusion body formation

IF 4.8 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbial Biotechnology Pub Date : 2022-07-13 DOI:10.1111/1751-7915.14117

Julian Kopp, Benjamin Bayer, Christoph Slouka, Gerald Striedner, Mark Dürkop, Oliver Spadiut

引用次数: 2

Abstract

Early-stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod-shaped protein aggregates, a potential sponge-like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod-shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations.

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早期包涵体形成的基本见解

早期包涵体的形成仍然是个谜。关于棒状蛋白质聚集体的存在，一种潜在的海绵状包涵体支架以及每个大肠杆菌细胞包涵体的数量，文献是模棱两可的。在这项研究中，我们证实了杆状包涵体的存在，证实了它们的多孔形态，每个细胞中存在多个蛋白质聚集体，并模拟了包涵体形成与代数的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY

CiteScore

9.80

自引率

3.50%

发文量

162

审稿时长

6-12 weeks

期刊介绍： Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes