Nephroprotective Potential of Sphaeranthus indicus Linn Extract against Hyperglycemia and Dyslipidemia in Streptozotocin-Induced Diabetic Nephropathy

IF 0.3 Q3 MEDICINE, GENERAL & INTERNAL
Vishalaxi Jadhav, J. Vaghela
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引用次数: 0

Abstract

Abstract Objective  This study was aimed at determining the nephroprotective potential of Sphaeranthus indicus Linn methanol extract (SME) against hyperglycemia and dyslipidemia in streptozotocin (STZ)-induced diabetic nephropathy (DNP) in adult Wistar albino rats. Materials and Methods  Following STZ-induced diabetes, adult albino Wistar rats of either sex with serum glucose level more than 250 mg/dL were chosen and randomized into six groups ( n  = 6 rats per group) and received the treatment as follows: Group I: Normal nondiabetic (ND) rats received a single intraperitoneal dose of citrate buffer in the same volume as STZ and 1% (w/v) carboxymethyl cellulose (CMC) per os (po), group II: diabetic (STZ) control rats received oral dosage of 1% (w/v) CMC, group III, IV and V: STZ + SME treated rats received a suspension of SME (100, 200, and 400 mg/kg, po) in 1% (w/v) CMC, and group VI: STZ + MET treated rats received metformin (500 mg/kg, po) as suspension in 1% (w/v) CMC. From 28th day to the 56th day of STZ injection, SME and MET were given for 28 days in the form of freshly prepared suspension. The impact of STZ-induced DNP was analyzed through the estimation of body weight, serum glucose, and hemoglobin A1c levels, renal functional parameters, the serum lipid profile, oxidative stress markers, and analysis of renal histoarchitecture. Result  Diabetic (STZ) control rats showed significant alterations in body weight, serum glucose and hemoglobin A1c levels, renal functional parameters, the serum lipid profile, oxidative stress markers, and renal histoarchitecture in contrast to normal ND rats. SME and MET treatment significantly reduced hyperglycemia-induced enhanced lipid profile and oxidative stress, normalized renal functional parameters, and restored renal histoarchitecture by reducing vacuolar degeneration of renal tubules in contrast to diabetic (STZ) control rats. These findings were attributed to SME's efficacy in DNP. Conclusion  In STZ-sensitized diabetic rats, SME retarded the progress of nephropathy. The observed nephroprotective potential of SME is ascribed to its hypoglycemic, hypolipidemic, and antioxidant activities.
白花提取物对链脲佐菌素所致糖尿病肾病患者高血糖和血脂异常的保护作用
摘要目的探讨白化大鼠链脲佐菌素(STZ)诱导的糖尿病肾病(DNP)的肾脏保护作用。材料与方法选择血清葡萄糖水平大于250 mg/dL的成年白化Wistar大鼠,随机分为6组(每组6只),分别给予如下治疗:1组:正常非糖尿病大鼠腹腔注射与STZ等量的柠檬酸缓冲液,每os (po)给予1% (w/v)羧甲基纤维素(CMC);糖尿病(STZ)对照大鼠口服剂量为1% (w/v) CMC, III、IV、v组:STZ + SME处理大鼠在1% (w/v) CMC中给予SME(100、200、400 mg/kg, po)混悬液,VI组:STZ + MET处理大鼠在1% (w/v) CMC中给予二甲双胍(500 mg/kg, po)混悬液。STZ注射第28天至第56天,SME和MET以新鲜配制的混悬液形式给予28天。通过体重、血糖、血红蛋白A1c水平、肾功能指标、血脂、氧化应激指标和肾脏组织结构分析,分析stz诱导DNP的影响。结果与正常ND大鼠相比,STZ组大鼠的体重、血糖、糖化血红蛋白水平、肾功能指标、血脂、氧化应激指标和肾脏组织结构均发生了显著变化。与糖尿病(STZ)对照大鼠相比,SME和MET治疗可显著降低高血糖诱导的血脂和氧化应激升高,使肾功能参数正常化,并通过减少肾小管空泡变性来恢复肾脏组织结构。这些发现归因于SME对DNP的疗效。结论在stz致敏的糖尿病大鼠中,SME延缓了肾病的进展。观察到的SME的肾保护潜力归因于其降血糖、降血脂和抗氧化活性。
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来源期刊
Journal of Health and Allied Sciences NU
Journal of Health and Allied Sciences NU MEDICINE, GENERAL & INTERNAL-
自引率
33.30%
发文量
85
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