Introgressive hybridization between two close speciesSiniperca chuatsiandSiniperca kneri(Percomorpharia: Sinipercidae) in the Middle Reaches of the Yangtze River

IF 1.5 4区农林科学 Q3 FISHERIES

Aquatic Living Resources Pub Date : 2021-01-01 DOI:10.1051/ALR/2021001

Shuqin Zhu, Chenhong Li, Jinliang Zhao

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引用次数: 1

Abstract

Siniperca chuatsiandSiniperca kneriare two economically important freshwater fishes endemic to East Asia. Recently, someSinipercaspecimens collected from Lake Poyang and Lake Dongting in the middle reaches of the Yangtze River couldn't be clearly identified as they showed intermediate morphological characteristics betweenS. chuatsiandS. kneri,and some inter-species hybrids were detected by microsatellite loci. To further verify genetic composition of these intermediate individuals, and determine the prevalence and degree of introgression between the twoSinipercaspecies, a large set of high-quality, independent, diagnostic genetic markers were necessarily required. Based on enrichment and sequencing of target genes in sinipercid fishes, 463 loci (FST = 1) betweenS. chuatsiandS. kneriwere selected and verified for species diagnosis. A total of 349 loci with 458 diagnostic SNPs were identified for discriminatingS. chuatsiandS. kneri. From those markers, 224 diagnostic SNPs (only one SNP per locus) were selected to identify and categorize 48 specimens with intermediate morphological characters. The results showed that there were 8 specimens identified as hybrids, 8 specimens asS. chuatsiand 32 specimens asS. kneri.NEWHYBRIDS analysis showed that the hybrid offsprings were composed of the first-generation hybrid (2 individuals), first-generation backcross (1 individual), second-generation backcross (1 individual) and fourth-generation backcross (4 individuals), and the backcrossing could happen to bothS. chuatsiandS. kneri. These hybrids could occur naturally, or escaped from farmed fish, due to extensive artificial breeding practice in these regions. However, the origin of the introgressive hybridization can't be easily traced. Therefore, some measures for protecting genetic resource ofSinipercaspeies in the Yangtze River should be enforced, such as assessing genetic background of the cultured stocks, reducing the escapement from farmed fish, and monitoring the trend of introgressive hybridization betweenSinipercaspecies in the future.

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长江中游两个近缘种川西鳜和克氏鳜的渐进杂交

中华鳜鱼(sininipera chuatsika)和中华鳜鱼(sininipera kneriia)是东亚特有的两种重要的淡水鱼。近年来，在长江中游鄱阳湖和洞庭湖采集的一些叶蝉标本，由于形态特征介于两者之间，不能很好地识别。chuatsiandS。微卫星位点检测到Kneri和一些种间杂交种。为了进一步验证这些中间个体的遗传组成，并确定这两个物种之间的遗传渗入率和程度，需要大量高质量、独立的诊断性遗传标记。通过对目标基因的富集和测序，发现了463个位点(FST = 1)。chuatsiandS。选择knerii进行菌种诊断验证。共有349个位点和458个诊断性snp被鉴定用于鉴别。chuatsiandS。kneri。从这些标记中，选择224个诊断SNP(每个位点仅1个SNP)对48个具有中间形态特征的标本进行鉴定和分类。结果表明，杂交品种8份，川岛小偃麦草8份，克纳利小偃麦草32份。NEWHYBRIDS分析表明，杂交后代由第一代杂交(2个个体)、第一代回交(1个个体)、第二代回交(1个个体)和第四代回交(4个个体)组成，两者都可能发生回交。chuatsiandS。kneri。由于这些地区广泛的人工繁殖，这些杂交品种可能自然发生，也可能从养殖鱼类中逃逸。然而，渐渗杂交的起源却不容易追溯。因此，今后应加强对养殖种群遗传背景的评估、减少养殖鱼类的逃逸、监测种属间渐渗杂交的趋势等措施，以保护长江江豚的遗传资源。

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来源期刊

Aquatic Living Resources 农林科学-海洋与淡水生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

>24 weeks

期刊介绍： Aquatic Living Resources publishes original research papers, review articles and propective notes dealing with all exploited (i.e. fished or farmed) living resources in marine, brackish and freshwater environments. Priority is given to ecosystem-based approaches to the study of fishery and aquaculture social-ecological systems, including biological, ecological, economic and social dimensions. Research on the development of interdisciplinary methods and tools which can usefully support the design, implementation and evaluation of alternative management strategies for fisheries and/or aquaculture systems at different scales is particularly welcome by the journal. This includes the exploration of scenarios and strategies for the conservation of aquatic biodiversity and research relating to the development of integrated assessment approaches aimed at ensuring sustainable and high quality uses of aquatic living resources.