{"title":"Simultaneous Determination of Hexoestrol, Diethylstilbestrol, Estrone and 17-Beta-estradiol in Feed by Gas Chromatography-mass Spectrometry","authors":"Huo Feng, Li Ning, Lin Xiao-Li","doi":"10.1016/S1006-8104(16)30031-9","DOIUrl":null,"url":null,"abstract":"<div><p>A method was developed for the simultaneous determination of four kinds of estrogens (hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol) in feed by gas chromatography-mass spectrometry (GC-MS). After the sample was extracted by ethyl ether and cleaned-up on HLB phase extraction column, four kinds of estrogens were derived and quantified in gas chromatography-mass spectrometry. The results showed that the linear detectable ranged from 2.5 ng · mL<sup>−1</sup> to 250 ng · mL<sup>−1</sup> for hexoestrol and from 5 ng · mL<sup>−1</sup> to 500 ng · mL<sup>−1</sup> for three other estrogens with the correlation coefficients (<em>R</em><sup>2</sup>) were no less than 0.990. The recoveries were in the range of 76.34%–96.33% and the relative standard deviation was no more than 22.7%. The limits of quantitation (LOQ) for all analytics were between 10 ug · kg<sup>−1</sup> and 20 ug · kg<sup>−1</sup>. The method was accurate and sensitive and could meet the actual requirements for the analyses of feed samples.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30031-9","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Northeast Agricultural UniversityEnglish Edition","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1006810416300319","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
A method was developed for the simultaneous determination of four kinds of estrogens (hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol) in feed by gas chromatography-mass spectrometry (GC-MS). After the sample was extracted by ethyl ether and cleaned-up on HLB phase extraction column, four kinds of estrogens were derived and quantified in gas chromatography-mass spectrometry. The results showed that the linear detectable ranged from 2.5 ng · mL−1 to 250 ng · mL−1 for hexoestrol and from 5 ng · mL−1 to 500 ng · mL−1 for three other estrogens with the correlation coefficients (R2) were no less than 0.990. The recoveries were in the range of 76.34%–96.33% and the relative standard deviation was no more than 22.7%. The limits of quantitation (LOQ) for all analytics were between 10 ug · kg−1 and 20 ug · kg−1. The method was accurate and sensitive and could meet the actual requirements for the analyses of feed samples.