Functional Mechanism of Resveratrol in Inhabiting Growth of Cells Is174t and Its Mechanism in Subcutaneously Transplanted Tumor of Nude Mice

IF 3.1 4区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Research in Chinese Universities Pub Date : 2008-11-01 DOI:10.1016/S1005-9040(09)60022-2

Jie CHEN, Xin-shu DONG, Xing-gang GUO

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引用次数: 2

Abstract

To explore the functional mechanism of Resveratrol against colon cancer cells Is174t and the growth of colon cancer tissue of tumor-bearing mice, MTT method was used to observe the functions of resveratrol for inhibition against cells Is174t in vitro. Transmission electron microscope was used to observe the cell apoptosis. FCM assay was performed to measure the change of the cell apoptosis rate and of cell cycle. RT-PCR method was used to detect the expressions of bcl-2 and bax mRNA. Western blot method was used to detect the expressions of bcl-2 and bax protein. Cells Is174t were transplanted subcutaneously to nude mice to observe the effect of resveratrol on the growth of subcutaneously transplanted tumor. RT-PCR method was used to detect the expressions of bcl-2 and bax mRNA in the tumor tissue. Western blot method was used to detect the expressions of bcl-2 and bax protein in the tumor tissue. Resveratrol has an effect of inhibiting proliferation of cells Is174t in vitro(P<0.01). It is able to induce the apoptosis of cells Is174t, causing the decrease in the expression of bcl-2 and the increase in the expression of bax. Resveratrol could inhibit the growth of subcutaneously transplanted tumor of nude mice(P<0.05), causing the decrease in the expression of bcl-2 and the increase in the expression of bax. Resveratrol can inhibit the growth of cells 174t and the growth of subcutaneously transplanted tumor. The mechanism is possibly related to the induction of the cell apoptosis and the regulation of bcl-2/bax expression.

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白藜芦醇在裸小鼠皮下移植瘤细胞Is174t驻留生长中的作用机制及其作用机制

为探讨白藜芦醇对结肠癌细胞Is174t及荷瘤小鼠结肠癌组织生长的作用机制，采用MTT法体外观察白藜芦醇对结肠癌细胞Is174t的抑制作用。透射电镜观察细胞凋亡情况。流式细胞仪检测细胞凋亡率和细胞周期的变化。采用RT-PCR法检测bcl-2和bax mRNA的表达。Western blot法检测bcl-2和bax蛋白的表达。裸鼠皮下移植细胞Is174t，观察白藜芦醇对皮下移植瘤生长的影响。采用RT-PCR法检测肿瘤组织中bcl-2和bax mRNA的表达。Western blot法检测肿瘤组织中bcl-2、bax蛋白的表达。白藜芦醇具有体外抑制细胞Is174t增殖的作用(P<0.01)。能诱导细胞Is174t凋亡，导致bcl-2表达降低，bax表达升高。白藜芦醇能抑制裸鼠皮下移植瘤的生长(P<0.05)，使bcl-2表达降低，bax表达升高。白藜芦醇能抑制细胞的生长和皮下移植瘤的生长。其机制可能与诱导细胞凋亡和调控bcl-2/bax的表达有关。

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来源期刊

Chemical Research in Chinese Universities 化学-化学综合

CiteScore

5.30

自引率

6.50%

发文量

152

审稿时长

3.0 months

期刊介绍： The journal publishes research articles, letters/communications and reviews written by faculty members, researchers and postgraduates in universities, colleges and research institutes all over China and overseas. It reports the latest and most creative results of important fundamental research in all aspects of chemistry and of developments with significant consequences across subdisciplines. Main research areas include (but are not limited to): Organic chemistry (synthesis, characterization, and application); Inorganic chemistry (bio-inorganic chemistry, inorganic material chemistry); Analytical chemistry (especially chemometrics and the application of instrumental analysis and spectroscopy); Physical chemistry (mechanisms, catalysis, thermodynamics and dynamics); Polymer chemistry and polymer physics (mechanisms, material, catalysis, thermodynamics and dynamics); Quantum chemistry (quantum mechanical theory, quantum partition function, quantum statistical mechanics); Biochemistry; Biochemical engineering; Medicinal chemistry; Nanoscience (nanochemistry, nanomaterials).