Isolation and Purification of Polysaccharides from Cordyceps minlitaris and Its Inhibition on the Proliferation of Rat Glomerular Mesangial Cells

IF 3.1 4区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
A-li HOU, Qing-fan MENG, Jin-shuang AN, Kai ZHU, Yun FENG, Li-rong TENG
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引用次数: 8

Abstract

The crude polysaccharide was obtained by means of the decolorization of porphyrized Cordyceps minlitaris stroma with organic solvent, extraction with hot water, precipitation in 80% ethanol, and protein removal with the Sevag method. After purification with Sephadex G-75, two of its components, CMP-1 and CMP-2, were obtained. Through the assay of gel chromatography and polarimetry, CMP-1 was identified as pure polysaccharide. The results demonstrated that CMP-1 had favorable oxidation resistance activity, which could scavenge not only oxygen-free radicals in the self-oxidation system of pyrogallic acid, but also the hydroxide-free radicals in the Fenton system. The study focused on the effects of low, medium, and high dosages of CMP-1 in rat blood serum on the proliferation of glomerular mesangial cells in vitro. Through MTT Colorimetric analysis, the activities were compared among the blank control group and the Niaoduqing positive control group CMP-1 and CMP-2. The results shows that CMP-1 was able to inhibit the proliferation of rat glomerular mesangial cells effectively. Therefore, CMP-1, one component of polysaccharides of Cordyceps minlitaris, was certainly a potential remedy for hyperplastic glomerular nephritis, whose antioxidant activity could slow down the process of chronic renal failure(CRF) to some extent.

冬虫夏草多糖的分离纯化及其对大鼠肾小球系膜细胞增殖的抑制作用
采用有机溶剂对卟啉化的冬虫夏草基质进行脱色,热水提取,80%乙醇沉淀,Sevag法去除蛋白质,得到粗多糖。用Sephadex G-75纯化后,得到CMP-1和CMP-2两种成分。经凝胶层析和极化法鉴定,CMP-1为纯多糖。结果表明,CMP-1具有良好的抗氧化活性,不仅能清除焦性没食子酸自氧化体系中的氧自由基,还能清除Fenton体系中的羟基自由基。研究大鼠血清低、中、高剂量CMP-1对体外培养肾小球系膜细胞增殖的影响。采用MTT比色法比较空白对照组与脑毒清阳性对照组CMP-1、CMP-2的活性。结果表明,CMP-1能有效抑制大鼠肾小球系膜细胞的增殖。因此,冬虫夏草多糖的一种成分CMP-1肯定是治疗增生性肾小球肾炎的潜在药物,其抗氧化活性可以在一定程度上减缓慢性肾衰竭的进程。
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来源期刊
CiteScore
5.30
自引率
6.50%
发文量
152
审稿时长
3.0 months
期刊介绍: The journal publishes research articles, letters/communications and reviews written by faculty members, researchers and postgraduates in universities, colleges and research institutes all over China and overseas. It reports the latest and most creative results of important fundamental research in all aspects of chemistry and of developments with significant consequences across subdisciplines. Main research areas include (but are not limited to): Organic chemistry (synthesis, characterization, and application); Inorganic chemistry (bio-inorganic chemistry, inorganic material chemistry); Analytical chemistry (especially chemometrics and the application of instrumental analysis and spectroscopy); Physical chemistry (mechanisms, catalysis, thermodynamics and dynamics); Polymer chemistry and polymer physics (mechanisms, material, catalysis, thermodynamics and dynamics); Quantum chemistry (quantum mechanical theory, quantum partition function, quantum statistical mechanics); Biochemistry; Biochemical engineering; Medicinal chemistry; Nanoscience (nanochemistry, nanomaterials).
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