RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene

Mutation Research/DNA Repair Pub Date : 2001-04-04 DOI:10.1016/S0921-8777(01)00061-1

Shirong Yu , Yumin Teng , Noel F. Lowndes , Raymond Waters

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引用次数: 19

Abstract

In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the MFA2 gene in haploid Saccharomyces cerevisiae (S. cerevisiae) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20 J/m² enhances the removal of CPDs induced by a second UV dose of 100 J/m² in the TS and the NTS of MFA2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the transcription coupled repair gene RAD26 are essential for inducible NER.

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在Saccharomyces cerevisiae MFA2基因的转录区和非转录区，紫外线诱导的环丁烷嘧啶二聚体的核苷酸切除修复需要RAD9、RAD24、RAD16和RAD26

本研究研究了紫外线照射对单倍体酿酒酵母细胞MFA2基因转录链(TS)和非转录链(NTS)上环丁烷嘧啶二聚体(CPDs)去除的影响。在NER感态细胞中，20 J/m2的预照射增强了MFA2基因TS和NTS中第二次100 J/m2的紫外线剂量诱导的CPDs的去除，但NTS中+258至+298区域的CPDs不存在增强修复。在rad9、rad24、rad16和rad26细胞中均未观察到诱导性修复，说明两个检查点基因rad9和rad24、全局修复基因rad16和转录偶联修复基因rad26是诱导性NER的必要条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mutation Research/DNA Repair

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