Comprehensive analysis of a large genomic sequence at the putative B-cell chronic lymphocytic leukaemia (B-CLL) tumour suppresser gene locus

Mutation Research/Mutation Research Genomics Pub Date : 2001-12-01 DOI:10.1016/S0027-5107(01)00219-6

Gaëlle Rondeau , Isabelle Moreau , Stéphane Bézieau , Jean-Louis Petit , Roland Heilig , Sylvaine Fernandez , Erwan Pennarun , Jeremy S. Myers , Mark A. Batzer , Jean-Paul Moisan , Marie-Claire Devilder

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引用次数: 29

Abstract

In many haematological diseases, and more particularly in B-cell chronic lymphocytic leukaemia (B-CLL), the existence of a tumour suppressor gene located within the frequently deleted region 13q14.3, has been put forward. A wide candidate region spanning from marker D13S273 to D13S25 has been proposed and an extensive physical map has been constructed by several teams. In this study, we sequenced a minimal core deleted region that we have previously defined and annotated it with flanking available public sequences. Our analysis shows that this region is gene-poor. Furthermore, our work allowed us to identify new alternative transcripts, spanning core regions, of the previously defined candidate genes DLEU1 and DLEU2. Since their putative involvement in B-CLL was controversial, our present study provide support for reconsidering the DLEU1 and DLEU2 genes as B-CLL candidate genes, with a new definition of their organisation and context.

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对假定的B细胞慢性淋巴细胞白血病（B-CLL）肿瘤抑制基因座的大基因组序列的综合分析。

在许多血液病中，特别是在b细胞慢性淋巴细胞白血病(B-CLL)中，已经提出了位于经常缺失的13q14.3区域的肿瘤抑制基因的存在。多个团队提出了从标记D13S273到D13S25的广泛候选区域，并构建了广泛的物理地图。在这项研究中，我们对一个最小的核心缺失区域进行了测序，我们之前已经定义了这个区域，并用其侧面的可用公共序列对其进行了注释。我们的分析表明这个区域是基因贫乏的。此外，我们的工作使我们能够识别新的替代转录本，跨越先前定义的候选基因DLEU1和DLEU2的核心区域。由于对其参与B-CLL的假设存在争议，我们目前的研究为重新考虑DLEU1和DLEU2基因作为B-CLL候选基因提供了支持，并对其组织和背景进行了新的定义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mutation Research/Mutation Research Genomics

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